Development of a split-luciferase assay to establish optimal protein secretion conditions for protein production by Bacillus subtilis

References

1. Harwood CR, Cranenburgh R. Bacillus protein secretion: an unfolding story. Trends Microbiol 2008; 16:73–79 [View Article] [PubMed]
   [Google Scholar]
2. Pohl S, Harwood CR. Heterologous Protein Secretion by Bacillus Species. from the Cradle to the Grave, 1st. edn Elsevier Inc; 2010
   [Google Scholar]
3. Cai D, Rao Y, Zhan Y, Wang Q, Chen S. Engineering Bacillus for efficient production of heterologous protein: current progress, challenge and prospect. J Appl Microbiol 2019; 126:1632–1642 [View Article] [PubMed]
   [Google Scholar]
4. Yoshida K, van Dijl JM. Engineering Bacillus subtilis cells as factories: enzyme secretion and value-added chemical production. Biotechnol Bioproc E 2020; 25:872–885 [View Article]
   [Google Scholar]
5. Maffei B, Francetic O, Subtil A. Tracking proteins secreted by bacteria: what’s in the toolbox?. Front Cell Infect Microbiol 2017; 7:221 [View Article] [PubMed]
   [Google Scholar]
6. Iizuka R, Yamagishi-Shirasaki M, Funatsu T. Kinetic study of de novo chromophore maturation of fluorescent proteins. Anal Biochem 2011; 414:173–178 [View Article] [PubMed]
   [Google Scholar]
7. Knapp A, Ripphahn M, Volkenborn K, Skoczinski P, Jaeger KE. Activity-independent screening of secreted proteins using split GFP. J Biotechnol 2017; 258:110–116 [View Article] [PubMed]
   [Google Scholar]
8. Cabantous S, Terwilliger TC, Waldo GS. Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nat Biotechnol 2005; 23:102–107 [View Article] [PubMed]
   [Google Scholar]
9. Müller C, Igwe CL, Wiechert W, Oldiges M. Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay. Microb Cell Fact 2021; 20:191 [View Article] [PubMed]
   [Google Scholar]
10. Santos-Aberturas J, Dörr M, Waldo GS, Bornscheuer UT. In-depth high-throughput screening of protein engineering libraries by split-GFP direct crude cell extract data normalization. Chem Biol 2015; 22:1406–1414 [View Article] [PubMed]
    [Google Scholar]
11. Van Engelenburg SB, Palmer AE. Imaging type-III secretion reveals dynamics and spatial segregation of Salmonella effectors. Nat Methods 2010; 7:325–330 [View Article] [PubMed]
    [Google Scholar]
12. Leonetti MD, Sekine S, Kamiyama D, Weissman JS, Huang B. A scalable strategy for high-throughput GFP tagging of endogenous human proteins. Proc Natl Acad Sci U S A 2016; 113:E3501-8 [View Article] [PubMed]
    [Google Scholar]
13. Lenz P, Bakkes PJ, Müller C, Malek M, Freudl R et al. Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay. Microb Cell Fact 2023; 22:203 [View Article] [PubMed]
    [Google Scholar]
14. Widder EA, Falls B. Review of bioluminescence for engineers and scientists in biophotonics. IEEE J Select Topics Quantum Electron 2014; 20:232–241 [View Article]
    [Google Scholar]
15. Fan F, Wood KV. Bioluminescent assays for high-throughput screening. Assay Drug Dev Technol 2007; 5:127–136 [View Article] [PubMed]
    [Google Scholar]
16. Westerhausen S, Nowak M, Torres-Vargas CE, Bilitewski U, Bohn E et al. A NanoLuc luciferase-based assay enabling the real-time analysis of protein secretion and injection by bacterial type III secretion systems. Mol Microbiol 2020; 113:1240–1254 [View Article] [PubMed]
    [Google Scholar]
17. Hall MP, Unch J, Binkowski BF, Valley MP, Butler BL et al. Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 2012; 7:1848–1857 [View Article] [PubMed]
    [Google Scholar]
18. Dixon AS, Schwinn MK, Hall MP, Zimmerman K, Otto P et al. NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS Chem Biol 2016; 11:400–408 [View Article] [PubMed]
    [Google Scholar]
19. Schwinn MK, Machleidt T, Zimmerman K, Eggers CT, Dixon AS et al. CRISPR-mediated tagging of endogenous proteins with a luminescent peptide. ACS Chem Biol 2018; 13:467–474 [View Article] [PubMed]
    [Google Scholar]
20. Pereira GC, Allen WJ, Watkins DW, Buddrus L, Noone D et al. A high-resolution luminescent assay for rapid and continuous monitoring of protein translocation across biological membranes. J Mol Biol 2019; 431:1689–1699 [View Article] [PubMed]
    [Google Scholar]
21. Hanahan D, Jessee J, Bloom FR. Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol 1991; 204:63–113 [View Article] [PubMed]
    [Google Scholar]
22. Spizizen J. Transformation of biochemically deficient strains of Bacillus subtilis by Deoxyribonucleate. Proc Natl Acad Sci U S A 1958; 44:1072–1078 [View Article] [PubMed]
    [Google Scholar]
23. Cruz RAL. A Comparative Study of Native and Heterologous Enzyme Production in Bacillus Subtilis Newcastle University; 2016
    [Google Scholar]
24. Bernier R, Desrochers M, Jurasek L, Paice MG. Isolation and characterization of a xylanase from Bacillus subtilis. Appl Environ Microbiol 1983; 46:511–514 [View Article] [PubMed]
    [Google Scholar]
25. Yamaguchi S, Jeenes DJ, Archer DB. Protein-glutaminase from Chryseobacterium proteolyticum, an enzyme that deamidates glutaminyl residues in proteins: purification, characterization and gene cloning. Eur J Biochem 2001; 268:1410–1421 [View Article] [PubMed]
    [Google Scholar]
26. Kubala MH, Kovtun O, Alexandrov K, Collins BM. Structural and thermodynamic analysis of the GFP:GFP-nanobody complex. Protein Sci 2010; 19:2389–2401 [View Article] [PubMed]
    [Google Scholar]
27. Dauter Z, Dauter M, Brzozowski AM, Christensen S, Borchert TV et al. X-ray structure of Novamyl, the five-domain “maltogenic” alpha-amylase from Bacillus stearothermophilus: maltose and acarbose complexes at 1.7A resolution. Biochemistry 1999; 38:8385–8392 [View Article] [PubMed]
    [Google Scholar]
28. Heinrich J, Drewniok C, Neugebauer E, Kellner H, Wiegert T. The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis. Microb Cell Fact 2019; 18:31 [View Article] [PubMed]
    [Google Scholar]
29. Fu LL, Xu ZR, Li WF, Shuai JB, Lu P et al. Protein secretion pathways in Bacillus subtilis: implication for optimization of heterologous protein secretion. Biotechnol Adv 2007; 25:1–12 [View Article]
    [Google Scholar]
30. Fu G, Liu J, Li J, Zhu B, Zhang D. Systematic screening of optimal signal peptides for secretory production of heterologous proteins in Bacillus subtilis. J Agric Food Chem 2018; 66:13141–13151 [View Article] [PubMed]
    [Google Scholar]
31. Volkenborn K, Kuschmierz L, Benz N, Lenz P, Knapp A et al. The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis. Microb Cell Fact 2020; 19:154 [View Article] [PubMed]
    [Google Scholar]
32. Bakkes PJ, Lenz P, Müller C, Bida A, Dohmen-Olma D et al. Biosensor-based optimization of cutinase secretion by Corynebacterium glutamicum. Front Microbiol 2021; 12:750150 [View Article] [PubMed]
    [Google Scholar]
33. Hemmerich J, Rohe P, Kleine B, Jurischka S, Wiechert W et al. Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum. Microb Cell Fact 2016; 15:208 [View Article] [PubMed]
    [Google Scholar]
34. Szewczyk M. Gid4 (human) cellular assay. Open Lab Notebooks; 2020 https://openlabnotebooks.org/gid4-human-cellular-assay/ accessed 29 June 2022