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Volume 169, Issue 10

Suppressor mutants demonstrate the metabolic plasticity of unsaturated fatty acid synthesis in PAO1 Open Access

Abstract

PAO1 has two aerobic pathways for synthesis of unsaturated fatty acids (UFAs), DesA and DesB plus the oxygen independent FabAB pathway. The DesA desaturase acts on saturated acyl chains of membrane phospholipid bilayers whereas the substrates of the DesB desaturase are thought to be long chain saturated acyl-CoA thioesters derived from exogeneous saturated fatty acids that are required to support DesB-dependent growth. Under suitable aerobic conditions either of these membrane-bound desaturates can support growth of strains lacking the oxygen independent FabAB pathway. We previously studied function of the desaturase of in a strain that required supplementation with a UFA for growth and noted bypass suppression of the strain that restored UFA synthesis. We report three genes encoding lipid metabolism proteins that give rise to suppressor strains that bypass loss of the DesA and oxygen independent FabAB pathways.

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  • Published Online:
Keyword(s): desaturase , desaturation , LptA , Pseudomonas and suppressors
Funding
This study was supported by the:
  • Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases (Award AI15650)
    • Principle Award Recipient: JohnE. Cronan
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-10-11
2024-09-15
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/content/journal/micro/10.1099/mic.0.001400
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Suppressor mutants demonstrate the metabolic plasticity of unsaturated fatty acid synthesis in Pseudomonas aeruginosa PAO1
Microbiology 169, 001400 (2023); https://doi.org/10.1099/mic.0.001400
/content/journal/micro/10.1099/mic.0.001400
/content/journal/micro/10.1099/mic.0.001400
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