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Volume 169, Issue 7

The transport of mannitol in is carried out by a broad-substrate polyol transporter SmoEFGK and is affected by the ability to transport and metabolize fructose Open Access

Abstract

The locus (orbitol annitol xidation) is found on the chromosome of ’s tripartite genome. Mutations at the locus reduce or abolish the ability of the bacterium to grow on several carbon sources, including sorbitol, mannitol, galactitol, -arabitol and maltitol. The contribution of the locus to the metabolism of these compounds has not been previously investigated. Genetic complementation of mutant strains revealed that is responsible for growth on sorbitol and galactitol, while restores growth on mannitol and -arabitol. Dehydrogenase assays demonstrate that SmoS and MtlK are NAD-dependent dehydrogenases catalysing the oxidation of their specific substrates. Transport experiments using a radiolabeled substrate indicate that sorbitol, mannitol and -arabitol are primarily transported into the cell by the ABC transporter encoded by . Additionally, it was found that a mutation in either , which is found in an operon that encodes the fructose ABC transporter, or a mutation in , which encodes fructose kinase, leads to the induction of mannitol transport.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ABC transporter , metabolism , polyol and Rhizobium
Funding
This study was supported by the:
  • Natural Sciences and Engineering Research Council of Canada (Award RGPIN-2018-04966)
    • Principle Award Recipient: IvanJ. Oresnik
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-07-28
2025-01-18
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/content/journal/micro/10.1099/mic.0.001371
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The transport of mannitol in Sinorhizobium meliloti is carried out by a broad-substrate polyol transporter SmoEFGK and is affected by the ability to transport and metabolize fructose
Microbiology 169, 001371 (2023); https://doi.org/10.1099/mic.0.001371
/content/journal/micro/10.1099/mic.0.001371
/content/journal/micro/10.1099/mic.0.001371
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