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Volume 169, Issue 8

Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogen Open Access

Abstract

is the dominant pathogen recovered from lung infection in people with cystic fibrosis. However, as an understudied pathogen there are knowledge gaps in relation to its population biology, phenotypic traits and useful model strains. A phylogenomic study of was undertaken using a total of 283 genomes, of which 73 were sequenced and 49 phenotypically characterized as part of this study. Average nucleotide identity analysis (ANI) and phylogenetic alignment of core genes demonstrated that the population separated into two distinct evolutionary clades, defined as lineage 1 (=58 genomes) and lineage 2 (=221 genomes). To examine the population biology of , a representative subgroup of 77 genomes (28 from the reference databases and the 49 novel short-read genome sequences) were selected based on multilocus sequence typing (MLST), isolation source and phylogenetic placement criteria. Comparative genomics was used to identify lineage-specific genes – in lineage 1 and in lineage 2 – and diagnostic PCRs targeting them were successfully developed. Phenotypic analysis of 49 representative strains showed considerable inter-strain variance, but the majority of the isolates tested were motile and capable of biofilm formation. A striking absence of protease activity was observed, but no lineage-specific phenotypic differences were demonstrated. Using phylogenomic and phenotypic criteria, three model CF strains were identified, BCC0084 (lineage 1), BCC1272 (lineage 2a) and BCC0033 lineage 2b, and their complete genome sequences determined. CF strains BCC0033 and BCC0084, and the environmental reference strain, ATCC 17616, were all capable of short-term survival within a murine lung infection model. By mapping the population biology, identifying lineage-specific PCRs and model strains, we provide much needed baseline resources for future studies of .

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  • Accepted:
  • Published Online:
Keyword(s): Burkholderia multivorans , cystic fibrosis , infection modelling , phenotype and phylogenomics
Funding
This study was supported by the:
  • Medical Research Council (Award MR/T030062/1)
    • Principal Award Recipient: ThomasR. Connor
  • Wellcome Trust (Award 204457/Z/16/Z)
    • Principal Award Recipient: DanielR. Neill
  • Cystic Fibrosis Foundation (Award MAHENT20G0)
    • Principal Award Recipient: EshwarMahenthiralingam
  • Cystic Fibrosis Trust (Award SRC 022)
    • Principal Award Recipient: EshwarMahenthiralingam
  • Medical Research Council (Award BV19101122)
    • Principal Award Recipient: KasiaM. Parfitt
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-08-01
2025-11-07

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/content/journal/micro/10.1099/mic.0.001366
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Identification of two distinct phylogenomic lineages and model strains for the understudied cystic fibrosis lung pathogen Burkholderia multivorans
Microbiology 169, 001366 (2023); https://doi.org/10.1099/mic.0.001366
/content/journal/micro/10.1099/mic.0.001366
/content/journal/micro/10.1099/mic.0.001366
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