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Volume 169, Issue 8

Septum site placement in – identification and characterisation of mycobacterial homologues of MinD Open Access

Graphical Abstract

Graphical Abstract

and are homologues of MinD, and play a role in mycobacterial divisome assembly.

Abstract

A major virulence trait of () is its ability to enter a dormant state within its human host. Since cell division is intimately linked to metabolic shut down, understanding the mechanism of septum formation and its integration with other events in the division pathway is likely to offer clues to the molecular basis of dormancy. The genome lacks obvious homologues of several conserved cell division proteins, and this study was aimed at identifying and functionally characterising mycobacterial homologues of the septum site specification protein MinD ( MinD). Sequence homology based analyses suggested that the genomes of both and the saprophyte () encode two putative MinD homologues and . Of these, were found to be the true homologues, through complementation of the mutant HL1, overexpression studies, and structural comparisons. and fully complemented the mini-cell phenotype of HL1, and over-expression of in led to cell elongation and a drastic decrease in c.f.u. counts, indicating its essentiality in cell-division. MSMEG_3743 displayed ATPase activity, consistent with its containing a conserved Walker A motif. Interaction of Rv1708 with the chromosome associated proteins ScpA and ParB, implied a link between its septum formation role, and chromosome segregation. Comparative structural analyses showed Rv1708 to be closer in similarity to Ec MinD than Rv3660c. In summary we identify Rv1708 and MSMEG_3743 to be homologues of MinD, adding a critical missing piece to the mycobacterial cell division puzzle.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ATPase , chromosome segregation , E.coli MinD , Mycobacteria and septum formation
Funding
This study was supported by the:
  • Council of Scientific and Industrial Research (CSIR), Govt. of India (Award BSC104)
    • Principle Award Recipient: TirumalaiR Raghunand
  • Department of Biotechnology (DBT), Govt. of India (Award BT/PR15411/BRB/10/924/2011)
    • Principle Award Recipient: TirumalaiR Raghunand
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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2023-08-01
2025-04-25
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/content/journal/micro/10.1099/mic.0.001359
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Septum site placement in Mycobacteria – identification and characterisation of mycobacterial homologues of Escherichia coli MinD
Microbiology 169, 001359 (2023); https://doi.org/10.1099/mic.0.001359
/content/journal/micro/10.1099/mic.0.001359
/content/journal/micro/10.1099/mic.0.001359
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