1887

Abstract

Microbes that have evolved to live on lignocellulosic biomass face unique challenges in the effective and efficient use of this material as food. The bacterium sp. ANA-3 has the potential to utilize arabinan and arabinoxylan, and uptake of the monosaccharide, -arabinose, derived from these polymers, is known to be mediated by a single ABC transporter. We demonstrate that the substrate binding protein of this system, GafA, binds specifically to -arabinofuranose, which is the rare furanose form of -arabinose found in lignocellulosic biomass. The structure of GafA was resolved to 1.7 Å and comparison to YtfQ (GafA) revealed binding site adaptations that confer specificity for furanose over pyranose forms of monosaccharides, while selecting arabinose over another related monosaccharide, galactose. The discovery of a bacterium with a natural predilection for a sugar found abundantly in certain lignocellulosic materials suggests an intimate connection in the enzymatic release and uptake of the sugar, perhaps to prevent other microbes scavenging this nutrient before it mutarotates to -arabinopyranose. This biological discovery also provides a clear route to engineer more efficient utilization of plant biomass components in industrial biotechnology.

Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/F0147591)
    • Principle Award Recipient: JudithHawkhead
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-03-15
2024-11-10
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