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Volume 168, Issue 11

Exploring functional interplay amongst efflux pumps Open Access

This article is part of the Antimicrobial Efflux collection.

Abstract

Bacterial efflux pumps exhibit functional interplay that can translate to additive or multiplicative effects on resistance to antimicrobial compounds. In diderm bacteria, two different efflux pump structural types – single-component inner membrane efflux pumps and cell envelope-spanning multicomponent systems – cooperatively export antimicrobials with cytoplasmic targets from the cell. Harnessing our recently developed efflux platform, which is built upon an extensively efflux-deficient strain of , here we explore interplay amongst a panel of diverse efflux pumps. Specifically, we assessed the effect of simultaneously expressing two efflux pump-encoding genes on drug resistance, including single-component inner membrane efflux pumps (MdfA, MdtK and EmrE), tripartite complexes (AcrAB, AcrAD, MdtEF and AcrEF), and the acquired TetA(C) tetracycline resistance pump. Overall, the expression of two efflux pump-encoding genes from the same structural type did not enhance resistance levels regardless of the antimicrobial compound or efflux pump under investigation. In contrast, a combination of the tripartite efflux systems with single-component pumps sharing common substrates provided multiplicative increases to antimicrobial resistance levels. In some instances, resistance was increased beyond the product of resistance provided by the two pumps individually. In summary, the developed efflux platform enables the isolation of efflux pump function, facilitating the identification of interactions between efflux pumps.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibiotic resistance , efflux pumps , functional interplay and physiological functions
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-11-01
2024-04-19
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Exploring functional interplay amongst Escherichia coli efflux pumps
Microbiology 168, 001261 (2022); https://doi.org/10.1099/mic.0.001261
/content/journal/micro/10.1099/mic.0.001261
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