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Volume 168, Issue 11

Interspecies killing activity of tailocins Open Access

Abstract

Tailocins are ribosomally synthesized bacteriocins, encoded by bacterial genomes, but originally derived from bacteriophage tails. As with both bacteriocins and phage, tailocins are largely thought to be species-specific with killing activity often assumed to be directed against closely related strains. Previous investigations into interactions between tailocin host range and sensitivity across phylogenetically diverse isolates of the phytopathogen have demonstrated that many strains possess intraspecific tailocin activity and that this activity is highly precise and specific against subsets of strains. However, here we demonstrate that at least one strain of USA011R, defies both expectations and current overarching dogma because tailocins from this strain possess broad killing activity against other agriculturally significant phytopathogens such as and as well as against the clinical human pathogen serovar Choleraesui. Moreover, we show that the full spectrum of this interspecific killing activity is not conserved across closely related strains with data suggesting that even if tailocins can target different species, they do so with different efficiencies. Our results reported herein highlight the potential for and phenotypic divergence of interspecific killing activity of tailocins and establish a platform for further investigations into the evolution of tailocin host range and strain specificity.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobials , phage-derived bacteriocins , Pseudomonas syringae and tailocins
Funding
This study was supported by the:
  • Arizona Department of Agriculture (Award SCBGP-19-24)
    • Principle Award Recipient: DavidA Baltrus
  • National Institute of Food and Agriculture (Award 2016-67014-24805)
    • Principle Award Recipient: DavidA Baltrus
  • Division of Integrative Organismal Systems (Award IOS 1856556)
    • Principle Award Recipient: DavidA Baltrus
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-11-07
2024-04-25
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Interspecies killing activity of Pseudomonas syringae tailocins
Microbiology 168, 001258 (2022); https://doi.org/10.1099/mic.0.001258
/content/journal/micro/10.1099/mic.0.001258
/content/journal/micro/10.1099/mic.0.001258
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