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Volume 168, Issue 9

Structural characterisation of methanogen pseudomurein cell wall peptide ligases homologous to bacterial MurE/F murein peptide ligases Open Access

Abstract

Archaea have diverse cell wall types, yet none are identical to bacterial peptidoglycan (murein). Methanogens and possess cell walls of pseudomurein, a structural analogue of murein. Pseudomurein differs from murein in containing the unique archaeal sugar -acetyltalosaminuronic acid instead of -acetylmuramic acid, β−1,3 glycosidic bonds in place of β−1,4 bonds and only -amino acids in the peptide cross-links. We have determined crystal structures of methanogen pseudomurein peptide ligases (termed pMurE) from (Mfer762) and (Mth734) that are structurally most closely related to bacterial MurE peptide ligases. The homology of the archaeal pMurE and bacterial MurE enzymes is clear both in the overall structure and at the level of each of the three domains. In addition, we identified two UDP-binding sites in Mfer762 pMurE, one at the exterior surface of the interface of the N-terminal and middle domains, and a second site at an inner surface continuous with the highly conserved interface of the three domains. Residues involved in ATP binding in MurE are conserved in pMurE, suggesting that a similar ATP-binding pocket is present at the interface of the middle and the C-terminal domains of pMurE. The presence of pMurE ligases in members of the Methanobacteriales and Methanopyrales, that are structurally related to bacterial MurE ligases, supports the idea that the biosynthetic origins of archaeal pseudomurein and bacterial peptidoglycan cell walls are evolutionarily related.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): methanogen , MurE , murein , peptide ligase , pMurE and pseudomurein
Funding
This study was supported by the:
  • Marsden Fund (Award AGR1301)
    • Principle Award Recipient: RonimusRon S.
  • Australian Synchrotron
    • Principle Award Recipient: Sutherland-SmithAndrew J.
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-09-30
2024-04-19
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Structural characterisation of methanogen pseudomurein cell wall peptide ligases homologous to bacterial MurE/F murein peptide ligases
Microbiology 168, 001235 (2022); https://doi.org/10.1099/mic.0.001235
/content/journal/micro/10.1099/mic.0.001235
/content/journal/micro/10.1099/mic.0.001235
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