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Volume 168, Issue 8

A unique global metabolic trait of CSV86: metabolism of aromatics over simple carbon sources and co-metabolism with organic acids Free

Abstract

Hierarchical utilization of substrate by microbes (utilization of simple carbon sources prior to complex ones) poses a major limitation to the efficient remediation of aromatic pollutants. Aromatic compounds, being complex and reduced in nature, appear to be a deferred choice as the carbon source in the presence of a plethora of simple organic compounds in the environment. The soil bacterium CSV86 displays a unique carbon source utilization hierarchy. It preferentially utilizes aromatics over glucose and co-metabolizes them with succinate or pyruvate (Basu ., 2006, , 72 : 22226–2230). In the present study, the substrate utilization hierarchy for strain CSV86 was tested for additional simple carbon sources such as glycerol, acetate, and tri-carboxylic acid (TCA) cycle intermediates like α-ketoglutarate and fumarate. When grown on a mixture of aromatics (benzoate or naphthalene) plus glycerol, the strain displayed a diauxic growth profile with significantly high activity of aromatic utilization enzymes (catechol 1,2- or 2,3-dioxygenase, respectively) in the first-log phase. This suggests utilization of aromatics in the first-log phase followed by glycerol in the second-log phase. On a mixture of an aromatic plus organic acid (acetate, α-ketoglutarate or fumarate), the strain displayed a monoauxic growth profile, indicating co-metabolism. Interestingly, the presence of glycerol, acetate, α-ketoglutarate or fumarate does not repress metabolism/utilization of the aromatic. Thus, the substrate utilization hierarchy of strain CSV86 is aromatics=organic acids>glucose/glycerol, which is unique as compared to other species, where degradation of aromatics is repressed by glycerol, glucose, acetate or organic acids, including TCA cycle intermediates. This novel substrate utilization hierarchy appears to be a global metabolic phenomenon in strain CSV86, thus implying it to be an ideal host for metabolic engineering as well as for its potential application in bioremediation.

  • Received:
  • Accepted:
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Keyword(s): aromatics , glycerol , organic acids , preferential utilization , Pseudomonas and substrate utilization hierarchy
© 2022 The Authors
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2022-08-04
2024-04-19
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A unique global metabolic trait of Pseudomonas bharatica CSV86T: metabolism of aromatics over simple carbon sources and co-metabolism with organic acids
Microbiology 168, 001206 (2022); https://doi.org/10.1099/mic.0.001206
/content/journal/micro/10.1099/mic.0.001206
/content/journal/micro/10.1099/mic.0.001206
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