RT Journal Article SR Electronic(1) A1 Falguera, Jan V. T. A1 Stratton, Kathryn J. A1 Bush, Matthew J. A1 Jani, Charul A1 Findlay, Kim C. A1 Schlimpert, Susan A1 Nodwell, Justin R.YR 2022 T1 DNA damage-induced block of sporulation in Streptomyces venezuelae involves downregulation of ssgB JF Microbiology, VO 168 IS 6 OP SP 001198 DO https://doi.org/10.1099/mic.0.001198 PB Microbiology Society, SN 1465-2080, AB DNA damage often causes an arrest of the cell cycle that provides time for genome integrity to be restored. In bacteria, the classical SOS DNA damage response leads to an inhibition of cell division resulting in temporarily filamentous growth. This raises the question as to whether such a response mechanism might similarly function in naturally filamentous bacteria such as Streptomyces. Streptomyces exhibit two functionally distinct forms of cell division: cross-wall formation in vegetative hyphae and sporulation septation in aerial hyphae. Here, we show that the genotoxic agent mitomycin C confers a block in sporulation septation in   Streptomyces venezuelae   in a mechanism that involves, at least in part, the downregulation of ssgB. Notably, this DNA damage response does not appear to block cross-wall formation and may be independent of canonical SOS and developmental regulators. We also show that the mitomycin C-induced block in sporulation can be partially bypassed by the constitutive expression of ssgB, though this appears to be largely limited to mitomycin C treatment and the resultant spore-like cells have reduced viability., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001198