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Volume 169, Issue 1

A global perspective on bacterial diversity in the terrestrial deep subsurface Open Access

Abstract

While recent efforts to catalogue Earth’s microbial diversity have focused upon surface and marine habitats, 12–20 % of Earth’s biomass is suggested to exist in the terrestrial deep subsurface, compared to ~1.8 % in the deep subseafloor. Metagenomic studies of the terrestrial deep subsurface have yielded a trove of divergent and functionally important microbiomes from a range of localities. However, a wider perspective of microbial diversity and its relationship to environmental conditions within the terrestrial deep subsurface is still required. Our meta-analysis reveals that terrestrial deep subsurface microbiota are dominated by and , probably as a function of the diverse metabolic strategies of these taxa. Evidence was also found for a common small consortium of prevalent and operational taxonomic units across the localities. This implies a core terrestrial deep subsurface community, irrespective of aquifer lithology, depth and other variables, that may play an important role in colonizing and sustaining microbial habitats in the deep terrestrial subsurface. An contamination-aware approach to analysing this dataset underscores the importance of downstream methods for assuring that robust conclusions can be reached from deep subsurface-derived sequencing data. Understanding the global panorama of microbial diversity and ecological dynamics in the deep terrestrial subsurface provides a first step towards understanding the role of microbes in global subsurface element and nutrient cycling.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , bacterial , deep subsurface and meta-analysis
Funding
This study was supported by the:
  • Natural Sciences and Engineering Research Council of Canada
    • Principle Award Recipient: AndréRodrigues Soares
  • Inyo Country, CA
    • Principle Award Recipient: AndréRodrigues Soares
  • US National Park Service
    • Principle Award Recipient: AndréRodrigues Soares
  • Nye County Nuclear Waste Repository Program Office (NWRPO)
    • Principle Award Recipient: AndréRodrigues Soares
  • Hydrodynamic Group, LLC
    • Principle Award Recipient: AndréRodrigues Soares
  • US Department of Energy’s Subsurface Biogeochemical Research Program (SBR)
    • Principle Award Recipient: AndréRodrigues Soares
  • National Research Network for Low Carbon Energy and Environment (NRN-LCEE)
    • Principle Award Recipient: A.C. Mitchell
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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A global perspective on bacterial diversity in the terrestrial deep subsurface
Microbiology 169, 001172 (2023); https://doi.org/10.1099/mic.0.001172
/content/journal/micro/10.1099/mic.0.001172
/content/journal/micro/10.1099/mic.0.001172
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