@article{mbs:/content/journal/micro/10.1099/mic.0.001150, author = "Okaro, Udoka and Mou, Sherry and Lenkoue, Geraldin and Williams, Janice A. and Bonagofski, Ari and Larson, Peter and Kumar, Raina and DeShazer, David", title = "A type IVB pilin influences twitching motility and in vitro adhesion to epithelial cells in Burkholderia pseudomallei", journal= "Microbiology", year = "2022", volume = "168", number = "3", pages = "", doi = "https://doi.org/10.1099/mic.0.001150", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001150", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "biofilm formation", keywords = "melioidosis", keywords = "type IV pili", eid = "001150", abstract = "Type IV pili are involved in adhesion, twitching motility, aggregation, biofilm formation and virulence in a variety of Gram-negative bacteria. Burkholderia pseudomallei, the causative agent of melioidosis and a Tier 1 biological select agent, is a Gram-negative bacterium with eight type IV pili-associated loci (TFP1 to TFP8). Most have not been fully characterized. In this study, we investigated BPSS2185, an uncharacterized TFP8 gene that encodes a type IVB pilus protein subunit. Using genetic deletion and complementation analysis in B. pseudomallei JW270, we demonstrate that BPSS2185 plays an important role in twitching motility and adhesion to A549 human alveolar epithelial cells. Compared to JW270, the JW270 ΔBPSS2185 mutant failed to display twitching motility and did not adhere to the epithelial cells. These phenotypes were partially reversed by the complementation of BPSS2185 in the mutant strain. The study also shows that BPSS2185 is expressed only during the onset of mature biofilm formation and at the dispersal of a biofilm, suggesting that the motility characteristic is required to form a biofilm. Our study is the first to suggest that the BPSS2185 gene in TFP8 contributes to twitching motility, adhesion and biofilm formation, indicating that the gene may contribute to B. pseudomallei virulence.", }