1887

Abstract

The ubiquitous unicellular eukaryote, , is known to play a role in the survival and dissemination of is the leading cause of bacterial foodborne gastroenteritis world-wide and is a major public health problem. The ability of to interact and potentially invade epithelial cells is thought to be key for disease development in humans. We examined grown under standard laboratory conditions, 11168H with that harvested from within (11168H) or (11168H), and compared their ability to invade different cell lines. harvested from within amoebae had a ~3.7-fold increase in invasiveness into T84 human epithelial cells and a striking ~11-fold increase for re-entry into cells. We also investigated the invasiveness and survivability of six diverse representative strains within spp., our results confirm that invasion and survivability is likely host-cell-dependent. Our survival assay data led us to conclude that spp. are a transient host for and that survival within amoebae pre-adapts and enhances subsequent cell invasion. This study provides new insight into interactions with amoebae and its increased invasiveness potential in mammalian hosts.

Funding
This study was supported by the:
  • Biotechnology and Biological Sciences Research Council (Award BB/R012504/1 constituent project BBS/E/F/000PR10349)
    • Principle Award Recipient: BrendanW. Wren
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-02-17
2024-10-05
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