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Abstract

Access to deep-sea sponges brings with it the potential to discover novel antimicrobial candidates, as well as novel cold- and pressure-adapted bacteria with further potential clinical or industrial applications. In this study, we implemented a combination of different growth media, increased pressure and high-throughput techniques to optimize recovery of isolates from two deep-sea hexactinellid sponges, and sp., in the first culture-based microbial analysis of these two sponges. Using 16S rRNA gene sequencing for isolate identification, we found a similar number of cultivable taxa from each sponge species as well as improved recovery of morphotypes from at 22–25 °C compared to other temperatures, which allows a greater potential for screening for novel antimicrobial compounds. Bacteria recovered under conditions of increased pressure were from the phyla , and , except at 4 %O/5 bar, when the phylum was not observed. Cultured isolates from both sponge species displayed antimicrobial activity against and .

Funding
This study was supported by the:
  • Plymouth University (Award NA)
    • Principle Award Recipient: PoppyJ Hesketh-Best
  • Society for Applied Microbiology (Award NA)
    • Principle Award Recipient: MatthewJ Koch
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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/content/journal/micro/10.1099/mic.0.001123
2021-12-13
2024-03-29
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