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Volume 167, Issue 11

Membrane lipid and osmolyte readjustment in the alkaliphilic micromycete under cold, heat and osmotic shocks Free

Abstract

Previously, we showed for the first time that alkaliphilic fungi, in contrast to alkalitolerant fungi, accumulated trehalose under extremely alkaline conditions, and we have proposed its key role in alkaliphilia. We propose that high levels of trehalose in the mycelium of alkaliphiles may promote adaptation not only to alkaline conditions, but also to other stressors. Therefore, we studied changes in the composition of osmolytes, and storage and membrane lipids under the action of cold (CS), heat (HS) and osmotic (OS) shocks in the obligate alkaliphilic micromycete . During adaptation to CS, an increase in the degree of unsaturation of phospholipids was observed while the composition of osmolytes, membrane and storage lipids remained the same. Under HS conditions, a twofold increase in the level of trehalose and an increase in the proportion of phosphatidylethanolamines were observed against the background of a decrease in the proportion of phosphatidic acids. OS was accompanied by a decrease in the amount of membrane lipids, while their ratio remained unchanged, and an increase in the level of polyols (arabitol and mannitol) in the fungal mycelium, which suggests their role for adaptation to OS. Thus, the observed consistency of the composition of membrane lipids suggests that trehalose can participate in adaptation not only to extremely alkaline conditions, but also to other stressors – HS, CS and OS. Taken together, the data obtained indicate the adaptability of the fungus to the action of various stressors, which can point to polyextremotolerance.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cold shock , heat shock , membrane lipids , osmolytes , osmotic shock and Sodiomyces tronii
Funding
This study was supported by the:
  • RFBR (Award № 18-04-00488)
    • Principle Award Recipient: VeraM. Tereshina
© 2021 The Authors
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2021-11-24
2024-04-18
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Membrane lipid and osmolyte readjustment in the alkaliphilic micromycete Sodiomyces tronii under cold, heat and osmotic shocks
Microbiology 167, 001112 (2021); https://doi.org/10.1099/mic.0.001112
/content/journal/micro/10.1099/mic.0.001112
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