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Volume 167, Issue 11

Influence of tissue and geographic locality on culturable endophytic bacteria of Free

Abstract

The endophytic bacterial community and their diversity are closely related to the host’s growth and development. This paper explores the culturable endophytic bacteria in the stems, leaves, roots and rhizomes of (AM) of four localities (Yuqian, Wenxian, Pan’an and Pingjiang) and the potential correlation between the bacteria and plant bioactive compounds. A total of 118 endophytic bacteria belonging to 3 phyla, 5 classes, 11 orders, 26 families and 48 genera were isolated and identified from the four AM tissues. Among them, was the dominant genus. In AM, the tissue type and locality influenced the endophytic bacterial community. Approximately 29.7 and 28.8% of the endophytic bacteria exhibited tissue specificity and geographic specificity, respectively. Furthermore, high-performance liquid chromatography revealed that the sesquiterpenoid (atractylenolide I, atractylenolide Ⅱ and atractylon) content was more in the rhizomes of Wenxian than in those of Pingjiang, Yuqian and Pan’an. The multiple linear regression was used to screen the bacterial strains related to the bioactive compounds of AM. The relative frequency of positively correlated with atractylenolide I and atractylon content in AM but negatively correlated with atractylenolide Ⅱ content. The study also provides a theoretical framework for future research on endophytic bacteria as alternative sources of secondary plant metabolites.

  • Received:
  • Accepted:
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Keyword(s): Atractylodes macrocephala , diversity , endophytic bacteria , locality and tissue
Funding
This study was supported by the:
  • Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences and Peking Union Medical College (Award 2021ZR09)
    • Principle Award Recipient: BoZhu
  • Natural Science Foundation of Zhejiang Province (Award LQ21H280003)
    • Principle Award Recipient: BoZhu
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 82003896)
    • Principle Award Recipient: BoZhu
© 2021 The Authors
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2021-11-26
2024-04-25
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References

  1. Zhu B, Zhang QL, Hua JW, Cheng WL, Qin LP. The traditional uses, phytochemistry, and pharmacology of Atractylodes macrocephala Koidz.: A review. J Ethnopharmacol 2018; 226:143–167 [View Article] [PubMed]
     [Google Scholar]
  2. Chen ZL. The Acetylenes from Atractylodes macrocephala. Planta Med 1987; 53:493–494 [View Article] [PubMed]
     [Google Scholar]
  3. Jia M, Chen L, Xin H-L, Zheng C-J, Rahman K et al. A friendly relationship between endophytic fungi and medicinal plants: A systematic review. Front Microbiol 2016; 7:906–920 [View Article] [PubMed]
     [Google Scholar]
  4. Zhao L, Xu Y, Lai XH, Shan C, Deng Z et al. Screening and characterization of endophytic Bacillus and Paenibacillus strains from medicinal plant Lonicera japonica for use as potential plant growth promoters. Braz J Microbiol 2015; 46:977–989 [View Article] [PubMed]
     [Google Scholar]
  5. Qin S, Miao Q, Feng W-W, Wang Y, Zhu X et al. Biodiversity and plant growth promoting traits of culturable endophytic actinobacteria associated with Jatropha curcas L. growing in Panxi dry-hot valley soil. Agric, Ecosyst Environ, Appl Soil Ecol 2015; 93:47–55 [View Article]
     [Google Scholar]
  6. Yang HR, Hu XP, Jiang CJ, Qi J, Wu YC et al. Diversity and antimicrobial activity of endophytic fungi isolated from Cephalotaxus hainanensis Li, a well-known medicinal plant in China. Lett Appl Microbiol 2015; 61:484–490 [View Article] [PubMed]
     [Google Scholar]
  7. Zhang GF, Huang QL, Bi XQ, Liu YL, Yuan ZS. Analysis of endophytic bacterial community diversity and metabolic correlation in Cinnamomum camphora. Arch Microbiol 2020; 202:181–189 [View Article] [PubMed]
     [Google Scholar]
  8. Yan Y, Zhang S, Zhang J, Ma P, Duan J et al. Effect and mechanism of endophytic bacteria on growth and secondary metabolite synthesis in Salvia miltiorrhiza hairy roots. Acta Physiol Plant 2014; 36:1095–1105 [View Article]
     [Google Scholar]
  9. Liu Y, Liu W, Liang Z. Endophytic bacteria from Pinellia ternata, a new source of purine alkaloids and bacterial manure. Pharm Biol 2015; 53:1545–1548 [View Article] [PubMed]
     [Google Scholar]
  10. Hong CE, Jo SH, Jo IH, Park JM. Diversity and antifungal activity of endophytic bacteria associated with Panax ginseng seedlings. Plant Biotechnol Rep 2018; 12:409–418 [View Article]
     [Google Scholar]
  11. Zhou JY, Yuan J, Li X, Ning YF, Dai CC. Endophytic bacterium-triggered reactive oxygen species directly increase oxygenous sesquiterpenoid content and diversity in Atractylodes lancea. Appl Environ Microbiol 2015; 82:1577–1585 [View Article] [PubMed]
     [Google Scholar]
  12. Li J, Zhao G-Z, Varma A, Qin S, Xiong Z et al. An endophytic Pseudonocardia species induces the production of artemisinin in Artemisia annua. PLoS One 2012; 7:e51410 [View Article] [PubMed]
     [Google Scholar]
  13. Afzal I, Iqrar I, Shinwari ZK, Yasmin A. Plant growth-promoting potential of endophytic bacteria isolated from roots of wild Dodonaea viscosa L. Plant Growth Regul 2016; 81:399–408 [View Article]
     [Google Scholar]
  14. Chen T, Chen Z, Ma GH, Du BH, Shen B et al. Diversity and potential application of endophytic bacteria in ginger. Genet Mol Res 2014; 13:4918–4931 [View Article]
     [Google Scholar]
  15. Herrera H, Sanhueza T, Novotná A, Charles TC, Arriagada C. Isolation and identification of endophytic bacteria from mycorrhizal tissues of Terrestrial Orchids from southern Chile. Diversity (Basel) 2020; 12:55 [View Article]
     [Google Scholar]
  16. Bredow C, Azevedo JL, Pamphile JA, Mangolin CA, Rhoden SA. In silico analysis of the 16S rRNA gene of endophytic bacteria, isolated from the aerial parts and seeds of important agricultural crops. Genet Mol Res 2015; 14:9703–9721 [View Article] [PubMed]
     [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article] [PubMed]
     [Google Scholar]
  18. Rivera-Orduña FN, Suarez-Sanchez RA, Flores-Bustamante ZR, Gracida-Rodriguez JN, Flores-Cotera LB. Diversity of endophytic fungi of Taxus globosa (Mexican yew). Fungal Divers 2010; 47:65–74 [View Article]
     [Google Scholar]
  19. Sadeghi F, Samsampour D, Seyahooei MA, Bagheri A, Soltani J. Diversity and spatiotemporal distribution of fungal endophytes associated with Citrus reticulata cv. siyahoo. Curr Microbiol 2019; 76:279–289 [View Article]
     [Google Scholar]
  20. Wu L, Han T, Li W, Jia M, Xue L et al. Geographic and tissue influences on endophytic fungal communities of Taxus chinensis var. mairei in China. Curr Microbiol 2013; 66:40–48 [View Article] [PubMed]
     [Google Scholar]
  21. Hu L, Chen X, Yang J, Guo L. Geographic authentication of the traditional Chinese medicine Atractylodes macrocephala Koidz. (Baizhu) using stable isotope and multielement analyses. Rapid Commun Mass Spectrom 2019; 33:1703–1710 [View Article] [PubMed]
     [Google Scholar]
  22. Wang K, Yan PS, Ding QL, Wu QX, Wang ZB et al. Diversity of culturable root-associated/endophytic bacteria and their chitinolytic and aflatoxin inhibition activity of peanut plant in China. World J Microbiol Biotechnol 2013; 29:1–10 [View Article] [PubMed]
     [Google Scholar]
  23. Hu X, Roberts DP, Xie L, Maul JE, Yu C et al. Formulations of Bacillus subtilis BY-2 suppress Sclerotinia sclerotiorum on oilseed rape in the field. Biol Control 2014; 70:54–64 [View Article]
     [Google Scholar]
  24. Zhao Y, Selvaraj JN, Xing F, Zhou L, Wang Y et al. Antagonistic action of Bacillus subtilis strain SG6 on Fusarium graminearum. PLoS One 2014; 9:e92486 [View Article] [PubMed]
     [Google Scholar]
  25. Hassan AHA, Hozzein WN, Mousa ASM, Rabie W, Alkhalifah DHM et al. Heat stress as an innovative approach to enhance the antioxidant production in Pseudooceanicola and Bacillus isolates. Sci Rep 2020; 10:15076 [View Article] [PubMed]
     [Google Scholar]
  26. Chen W, Fisher MJ, Leung A, Cao Y, Wong LL. Oxidative diversification of steroids by nature-inspired scanning glycine mutagenesis of P450BM3 (CYP102A1). ACS Catal 2020; 10:8334–8343 [View Article]
     [Google Scholar]
  27. Xing S-C, Chen J-Y, Chen Y-X, Wu R-T, Huang C-B et al. The combination of lead and Bacillus coagulans R11 increased the concentration of Alpha-Solanine in the cecum of laying hens and the pathogens abundance decreased. Front Microbiol 2020; 11:585197 [View Article] [PubMed]
     [Google Scholar]
  28. Yin DD, Wang YL, Yang M, Yin DK, Wang GK et al. Analysis of chuanxiong rhizoma substrate on production of ligustrazine in endophytic Bacillus subtilis by ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. J Sep Sci 2019; 42:3067–3076 [View Article] [PubMed]
     [Google Scholar]
  29. Song X, Wu H, Yin Z, Lian M, Yin C. Endophytic bacteria isolated from Panax ginseng improves ginsenoside accumulation in adventitious Ginseng root culture. Molecules 2017; 22:837–849 [View Article] [PubMed]
     [Google Scholar]
  30. Hashem A, Tabassum B, Fathi Abd Allah E. Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi J Biol Sci 2019; 26:1291–1297 [View Article] [PubMed]
     [Google Scholar]
  31. Mushtaq S, Faiza Khan MS, Hussain M, Haider MS. Endophytic Bacteria: A beneficial organism. t J Biosci 2017; 10:1–12 [View Article]
     [Google Scholar]
  32. Yuan ZS, Liu F, Zhang GF. Isolation of culturable endophytic bacteria from Moso bamboo (Phyllostachys edulis) and 16S rDNA diversity analysis. Arch Biol Sci 2015; 67:1001–1008 [View Article]
     [Google Scholar]
  33. Ma B, Lv X, Warren A, Gong J. Shifts in diversity and community structure of endophytic bacteria and archaea across root, stem and leaf tissues in the common reed, Phragmites australis, along a salinity gradient in a marine tidal wetland of northern China. Antonie van Leeuwenhoek 2013; 104:759–768 [View Article] [PubMed]
     [Google Scholar]
  34. Ma B, Lv X, Warren A, Gong J. Shifts in diversity and community structure of endophytic bacteria and archaea across root, stem and leaf tissues in the common reed, Phragmites australis, along a salinity gradient in a marine tidal wetland of northern China. Antonie van Leeuwenhoek 2013; 104:759–768 [View Article] [PubMed]
     [Google Scholar]
  35. Birgit M. Angela sessitsch advances in elucidating beneficial interactions between plants, soil, and bacteria. Adv Agron 2013 [View Article]
     [Google Scholar]
  36. Rasche F, Hödl V, Poll C, Kandeler E, Gerzabek MH et al. Rhizosphere bacteria affected by transgenic potatoes with antibacterial activities compared with the effects of soil, wild-type potatoes, vegetation stage and pathogen exposure. FEMS Microbiol Ecol 2006; 56:219–235 [View Article] [PubMed]
     [Google Scholar]
  37. Xu W, Wang F, Zhang M, Ou T, Wang R et al. Diversity of cultivable endophytic bacteria in mulberry and their potential for antimicrobial and plant growth-promoting activities. Microbiol Res 2019; 229:126328 [View Article] [PubMed]
     [Google Scholar]
  38. Zhou JY, Li X, Zhao D, Deng-Wang MY, Dai CC. Reactive oxygen species and hormone signaling cascades in endophytic bacterium induced essential oil accumulation in Atractylodes lancea. Planta 2016; 244:699–712 [View Article] [PubMed]
     [Google Scholar]
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Influence of tissue and geographic locality on culturable endophytic bacteria of Atractylodes macrocephala
Microbiology 167, 001109 (2021); https://doi.org/10.1099/mic.0.001109
/content/journal/micro/10.1099/mic.0.001109
/content/journal/micro/10.1099/mic.0.001109
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