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Abstract

the causative agent of cholera, uses a large number of coordinated transcriptional regulatory events to transition from its environmental reservoir to the host intestine, which is its preferred colonization site. Transcription of the mannose-sensitive haemagglutinin pilus (MSHA), which aids the persistence of in aquatic environments, but causes its clearance by host immune defenses, was found to be regulated by a yet unknown mechanism during the infection cycle of . In this study, genomic expression library screening revealed that two regulators, VC1371 and VcRfaH, are able to positively activate the transcription of MSHA operon. VC1371 is localized and active in the cell membrane. Deletion of or genes in resulted in less MshA protein production and less efficiency of biofilm formation compared to that in the wild-type strain. An adult mouse model showed that the mutants with or deletion colonized less efficiently than the wild-type; the deletion mutant showed less colonization efficiency in the infant mouse model. The findings strongly suggested that the two regulators, namely VC1371 and VcRfaH, which are involved in the regulation of MSHA expression, play an important role in biofilm formation and colonization in mice.

Funding
This study was supported by the:
  • natural science foundation of zhejiang province (Award LZ20C010001)
    • Principle Award Recipient: MenghuaYang
  • national natural science foundation of china (Award 31770151)
    • Principle Award Recipient: MenghuaYang
  • scientific research foundation of zhejiang a and f university (Award 2013FR012)
    • Principle Award Recipient: MenghuaYang
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2021-10-19
2024-12-12
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