Full text loading...
Abstract
Bacillus cereus is recognized as a causative agent of gastrointestinal syndromes, but can also cause a devastating form of intraocular infection known as endophthalmitis. We have previously reported that the PlcR/PapR master virulence factor regulator system regulates intraocular virulence, and that the S-layer protein (SlpA) contributes to the severity of B. cereus endophthalmitis. To better understand the role of other B. cereus virulence genes in endophthalmitis, expression of a subset of factors was measured at the midpoint of disease progression in a murine model of endophthalmitis by RNA-Seq. Several cytolytic toxins were expressed at significantly higher levels in vivo than in BHI. The virulence regulators codY, gntR, and nprR were also expressed in vivo. However, at this timepoint, plcR/papR was not detectable, although we previously reported that a B. cereus mutant deficient in PlcR was attenuated in the eye. The motility-related genes fla, fliF, and motB, and the chemotaxis-related gene cheA were detected during infection. We have shown previously that motility and chemotaxis phenotypes are important in B. cereus endophthalmitis. The sodA2 variant of manganese superoxide dismutase was the most highly expressed gene in vivo. Expression of the surface layer protein gene, slpA, an activator of Toll-like receptors (TLR)−2 and −4, was also detected during infection, albeit at low levels. Genes expressed in a mouse model of Bacillus endophthalmitis might play crucial roles in the unique virulence of B. cereus endophthalmitis, and serve as candidates for novel therapies designed to attenuate the severity of this often blinding infection.
- Received:
- Accepted:
- Published Online:
Funding
-
Research to Prevent Blindness
(Award Unrestricted grant to the Dean A. McGee Eye Institute)
- Principle Award Recipient: NotApplicable
-
Presbyterian Health Foundation
(Award Equipment Grant)
- Principle Award Recipient: MichelleC. Callegan
-
Presbyterian Health Foundation
(Award Research Support Grant)
- Principle Award Recipient: MichelleC. Callegan
-
National Institutes of Health
(Award P30EY027125)
- Principle Award Recipient: MichelleC. Callegan
-
National Institutes of Health
(Award R21EY028066)
- Principle Award Recipient: MichelleC. Callegan
-
National Institutes of Health
(Award R01EY028810)
- Principle Award Recipient: MichelleC. Callegan
-
National Institutes of Health
(Award R01EY024140)
- Principle Award Recipient: MichelleC. Callegan