RT Journal Article SR Electronic(1) A1 Calvelo, Vanessa Yoon A1 Crisante, David A1 Elliot, Marie A1 Nodwell, Justin ReaYR 2021 T1 The ARC2 response in Streptomcyes coelicolor requires the global regulatory genes afsR and afsS JF Microbiology, VO 167 IS 5 OP SP 001047 DO https://doi.org/10.1099/mic.0.001047 PB Microbiology Society, SN 1465-2080, AB ARC2 is a synthetic compound, related in structure and mechanism to the antibiotic triclosan, that activates the production of many specialized metabolites in the Streptomyces genus of bacteria. In this work, we demonstrate that the addition of ARC2 to Streptomyces coelicolor cultures results in considerable alterations in overall gene expression including most notably the specialized metabolic genes. Using actinorhodin production as a model system, we show that the effect of ARC2 depends on the pleiotropic regulators afsR and afsS but not afsK. We find that the constitutive expression of afsS can bypass the need for afsR but not the reverse, while the constitutive expression of afsK had no effect on actinorhodin production. These data are consistent with a model in which ARC2 activates a cell stress response that depends on AfsR activating the expression of the afsS gene such that AfsS then triggers the production of actinorhodin., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001047