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Volume 167, Issue 2

Atypical chlamydoconidium-producing strains from Ceará State, Northeast Brazil: investigation of taxonomy by phylogenetic analysis and biofilm susceptibility Free

Abstract

Chlamydoconidium-producing strains isolated in Northeastern Brazil have morphological features different from the classic description of this dermatophyte species. This study investigated the phylogenetic relationship of chlamydoconidium-producing strains isolated in Northeastern Brazil. Also, the effect of terbinafine and farnesol on mature biofilms of strains was evaluated. The mass spectra of strains were investigated by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The ITS and LSU loci regions of rDNA and the partial β-tubulin gene were sequenced and the phylogenetic tree was analysed. The effects of terbinafine and farnesol on mature biofilms were evaluated through the analysis of metabolic activity, quantification of biomass and observation by scanning electron microscopy. MALDI-TOF MS spectra of the chlamydoconidium-producing strains differed from the spectrum of the control strain (ATCC 28942), presenting an intense ion peak at 4155 Da. Phylogenetic tree analysis showed that the chlamydoconidium-producing strains isolated in Northeastern Brazil are allocated to a single cluster, differing from strains isolated from other countries. As for mature biofilms, farnesol reduced biomass and metabolic activity by 64.4 and 65.9 %, respectively, while terbinafine reduced the biomass by 66.5 % and the metabolic activity by 69 %. Atypical morphological characteristics presented by chlamydoconidium-producing strains result from phenotypic plasticity, possibly for adaptation to environmental stressors. Also, farnesol had inhibitory activity against biofilms, demonstrating this substance can be explored for development of promising anti-biofilm drugs against dermatophytes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , chlamydoconidia , farnesol , mass spectrometry , T. tonsurans and terbinafine
Funding
This study was supported by the:
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 306976/2017-0)
    • Principle Award Recipient: Josesidrm
© 2021 The Authors
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2021-01-11
2024-04-19
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Atypical chlamydoconidium-producing Trichophyton tonsurans strains from Ceará State, Northeast Brazil: investigation of taxonomy by phylogenetic analysis and biofilm susceptibility
Microbiology 167, 001018 (2021); https://doi.org/10.1099/mic.0.001018
/content/journal/micro/10.1099/mic.0.001018
/content/journal/micro/10.1099/mic.0.001018
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