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Volume 167, Issue 2

Point mutation in the stop codon of increases the growth rate of subspecies Open Access

Abstract

subspecies (MAH) is a pathogen that causes various non-tuberculous mycobacterial diseases in humans and animals worldwide. Among the genus, MAH is characterized by relatively slow growth. Here, we isolated a rapidly growing variant of the MAH 104 strain. The variant strain (named N104) exhibited an enhanced growth rate and higher motility compared to the parent MAH 104 strain (P104). Whole-genome sequencing analysis of N104 revealed the loss of the stop codon of due to a single nucleotide replacement, resulting in the substitution of the codon for tryptophan. Notably, exclusion of the stop codon ligated the open reading frames and caused the fusion of two adjacent proteins. A revertant parent strain, in which a mutation was introduced to restore the stop codon, revealed that elimination of the stop codon in was responsible for the N104 phenotype. Furthermore, we analysed the phenotypes of the parent and mutated strains by determining the functions of the and coding regions flanking the stop codon. The mutant strains, expected to express a fusion protein, exhibited increased resistance to antimicrobial drugs and exogenous copper toxicity compared to that of the parent strains. These findings suggest that the fusion of the - and -encoding regions in the mutant N104 strain could be related to the modified functions of these intrinsic proteins.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): growth rate , Mycobacterium avium subspecies hominissuis , single nucleotide replacement and sliding motility
Funding
This study was supported by the:
  • Japan Agency for Medical Research and Development (Award JP20fk0108129)
    • Principle Award Recipient: ManabuAto
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-12-23
2024-04-19
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Point mutation in the stop codon of MAV_RS14660 increases the growth rate of Mycobacterium avium subspecies hominissuis
Microbiology 167, 001007 (2021); https://doi.org/10.1099/mic.0.001007
/content/journal/micro/10.1099/mic.0.001007
/content/journal/micro/10.1099/mic.0.001007
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