1887

Abstract

infects patients with cystic fibrosis, burns, wounds and implants. Previously, our group showed that elevated Ca positively regulates the production of several virulence factors in , such as biofilm formation, production of pyocyanin and secreted proteases. We have identified a Ca-regulated β-propeller putative phytase, CarP, which is required for Ca tolerance, regulation of the intracellular Ca levels, and plays a role in Ca regulation of virulence. Here, we studied the conservation of sequence and its occurrence in diverse phylogenetic groups of bacteria. analysis revealed that and its two paralogues PA2017 and PA0319 are primarily present in and belong to the core genome of the species. We identified 155 single nucleotide alterations within , 42 of which lead to missense mutations with only three that affected the predicted 3D structure of the protein. PCR analyses with -specific primers detected specifically in 70 clinical and environmental samples. Sequence comparison demonstrated that is overall highly conserved in isolated from diverse environments. Such evolutionary preservation of illustrates its importance for adaptations to diverse environments and demonstrates its potential as a biomarker.

Funding
This study was supported by the:
  • MariannaA Patrauchan , National Institute of General Medical Sciences , (Award P20GM103648)
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/content/journal/micro/10.1099/mic.0.001004
2020-12-09
2021-02-26
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