@article{mbs:/content/journal/micro/10.1099/mic.0.001003, author = "Kloos, Julia and Johnsen, Pål J. and Harms, Klaus", title = "Tn1 transposition in the course of natural transformation enables horizontal antibiotic resistance spread in Acinetobacter baylyi", journal= "Microbiology", year = "2021", volume = "167", number = "1", pages = "", doi = "https://doi.org/10.1099/mic.0.001003", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.001003", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "transposition", keywords = "natural transformation", keywords = "Tn1", keywords = "Tn4401", keywords = "Acinetobacter baylyi", eid = "001003", abstract = "Transposons are genetic elements that change their intracellular genomic position by transposition and are spread horizontally between bacteria when located on plasmids. It was recently discovered that transposition from fully heterologous DNA also occurs in the course of natural transformation. Here, we characterize the molecular details and constraints of this process using the replicative transposon Tn1 and the naturally competent bacterium Acinetobacter baylyi . We find that chromosomal insertion of Tn1 by transposition occurs at low but detectable frequencies and preferably around the A. baylyi terminus of replication. We show that Tn1 transposition is facilitated by transient expression of the transposase and resolvase encoded by the donor DNA. RecA protein is essential for the formation of a circular, double-stranded cytoplasmic intermediate from incoming donor DNA, and RecO is beneficial but not essential in this process. Absence of the recipient RecBCD nuclease stabilizes the double-stranded intermediate. Based on these results, we suggest a mechanistic model for transposition during natural transformation.", }