RT Journal Article SR Electronic(1) A1 Söderström, Bill A1 Ruda, Alessandro A1 Widmalm, Göran A1 Daley, Daniel O.YR 2020 T1 An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy JF Microbiology, VO 166 IS 12 SP 1129 OP 1135 DO https://doi.org/10.1099/mic.0.000996 PB Microbiology Society, SN 1465-2080, AB Fluorescent d-amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an OregonGreen488-labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Gram-positive and some Gram-negative bacteria, and imaged by super-resolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000996