1887

Abstract

Fluorescent -amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an OregonGreen488-labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Gram-positive and some Gram-negative bacteria, and imaged by super-resolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm.

Funding
This study was supported by the:
  • Knut och Alice Wallenbergs Stiftelse
    • Principle Award Recipient: GöranWidmalm
  • Vetenskapsrådet (Award 2017-03703)
    • Principle Award Recipient: GöranWidmalm
  • Japan Society for the Promotion of Science (Award JP17K15694)
    • Principle Award Recipient: BillSöderström
  • Vetenskapsrådet
    • Principle Award Recipient: DanielDaley
  • Carl Tryggers Stiftelse för Vetenskaplig Forskning (SE)
    • Principle Award Recipient: DanielDaley
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/content/journal/micro/10.1099/mic.0.000996
2020-11-25
2021-08-02
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