1887

Abstract

A formylglycine-generating enzyme (FGE)–sulfatase-based whole-cell biosensor was genetically improved into a single-copy system by integrating the transcriptional activator ChpR and the promoter–FGE–sulfatase fusion into the chromosome. The sensitivity was further enhanced through a random mutagenesis of the . The new integrated biosensor offered both a lower detection limit [5 nM chlorpyrifos (CPF)] and fluorescence background. The ready-to-use kit was developed using silica gel for on-field detection. The biosensor kit was stable for 20 days when stored at 4 °C. Moreover, a 1-(1-naphthylmethyl)-piperazine (NMP) efflux pump inhibitor can improve the sensitivity by 57 %.

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/content/journal/micro/10.1099/mic.0.000979
2020-10-27
2021-10-22
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