Borrelia burgdorferi, a causative agent of Lyme disease, encodes a protein BBB07 on the genomic plasmid cp26. BBB07 was identified as a candidate integrin ligand based on the presence of an RGD tripeptide motif, which is present in a number of mammalian ligands for β1 and β3 integrins . Previous work demonstrated that BBB07 in recombinant form binds to β1 integrins and induces inflammatory responses in synovial cells in culture. Several transposon mutants in bbb07 were attenuated in an in vivo screen of the transposon library in mice. We therefore tested individual transposon mutant clones in single-strain infections in mice and found that they were attenuated in terms of ID50 but did not have significantly reduced tissue burdens in mice. Based on data presented here we conclude that BBB07 is not essential for, but does contribute to, B. burgdorferi infectivity in mice.
BeheraAK,
HildebrandE,
UematsuS,
AkiraS,
CoburnJ et al. Identification of a TLR-independent pathway for Borrelia burgdorferi-induced expression of matrix metalloproteinases and inflammatory mediators through binding to integrin α3β1. J Immunol2006; 177:657–664 [View Article][PubMed]
BeheraAK,
DurandE,
CuginiC,
AntonaraS,
BourassaL et al.Borrelia burgdorferi BBB07 interaction with integrin α3β1 stimulates production of pro-inflammatory mediators in primary human chondrocytes. Cell Microbiol2008; 10:320–331 [View Article][PubMed]
LinT,
GaoL,
ZhangC,
OdehE,
JacobsMB et al. Analysis of an ordered, comprehensive STM mutant library in infectious Borrelia burgdorferi: insights into the genes required for mouse infectivity. PLoS One2012; 7:e47532 [View Article][PubMed]
RistowLC,
BondeM,
LinYP,
SatoH,
CurtisM et al. Integrin binding by Borrelia burgdorferi P66 facilitates dissemination but is not required for infectivity. Cell Microbiol2015; 17:1021–1036 [View Article][PubMed]
RistowLC,
MillerHE,
PadmoreLJ,
ChettriR,
SalzmanN et al. The β3-integrin ligand of Borrelia burgdorferi is critical for infection of mice but not ticks. Mol Microbiol2012; 85:1105–1118 [View Article][PubMed]
MorrisonTB,
MaY,
WeisJH,
WeisJJ.
Rapid and sensitive quantification of Borrelia burgdorferi-infected mouse tissues by continuous fluorescent monitoring of PCR. J Clin Microbiol1999; 37:987–992 [View Article][PubMed]
ChanK,
AwanM,
BartholdSW,
ParveenN.
Comparative molecular analyses of Borrelia burgdorferi sensu stricto strains B31 and N40D10/E9 and determination of their pathogenicity. BMC Microbiol2012; 12:157 [View Article][PubMed]
TillyK,
GrimmD,
BueschelDM,
KrumJG,
RosaP.
Infectious cycle analysis of a Borrelia burgdorferi mutant defective in transport of chitobiose, a tick cuticle component. Vector Borne Zoonotic Dis2004; 4:159–168 [View Article][PubMed]