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Volume 166, Issue 9

Ciprofloxacin-induced persister-cells in Free

Abstract

is a major bacterial foodborne-pathogen. Ciprofloxacin is an important antibiotic for the treatment of , albeit high rates of fluoroquinolone resistance have limited its usefulness. Persister-cells are transiently antibiotic-tolerant fractions of bacterial populations and their occurrence has been associated with recalcitrant and persistent bacterial infections. Here, time-kill assays with ciprofloxacin (200×MIC, 25 µg ml) were performed in strains 81–176 and RM1221 and persister-cells were found. The frequency of survivors after 8 h of ciprofloxacin exposure was approx. 10 for both strains, while after 22 h the frequency was between 10–10, depending on the strain and growth-phase. Interestingly, the stationary-phase cultures did not display more persister-cells compared to exponential-phase cultures, in contrast to what has been observed in other bacterial species. Persister-cells after ampicillin exposure (100×MIC, 200 µg ml) were not detected, implying that persister-cell formation in is antibiotic-specific. In attempts to identify the mechanism of ciprofloxacin persister-cell formation, stringent or SOS responses were not found to play major roles. Overall, this study reports ciprofloxacin persister-cells in and challenges the notion of persister-cells as plainly dormant non-growing cells.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibiotic resistance , Campylobacter jejuni , ciprofloxacin and persister cells
© 2020 The Authors
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2020-07-22
2024-04-20
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Ciprofloxacin-induced persister-cells in Campylobacter jejuni
Microbiology 166, 849 (2020); https://doi.org/10.1099/mic.0.000953
/content/journal/micro/10.1099/mic.0.000953
/content/journal/micro/10.1099/mic.0.000953
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