@article{mbs:/content/journal/micro/10.1099/mic.0.000952, author = "Pickford, Hayleah and Alcock, Emily and Singh, Albel and Kelemen, Gabriella and Bhatt, Apoorva", title = "A mycobacterial DivIVA domain-containing protein involved in cell length and septation", journal= "Microbiology", year = "2020", volume = "166", number = "9", pages = "817-825", doi = "https://doi.org/10.1099/mic.0.000952", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000952", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "polar growth", keywords = "tuberculosis", keywords = "Mycobacterium", keywords = "cytoskeleton", keywords = "division", keywords = "septation", abstract = "Mycobacterial cells elongate via polar deposition of cell wall material, similar to the filamentous Streptomyces species, which contain a tip-organizing centre. Coiled-coiled proteins such as DivIVA play an important role in this process. The genome of Mycobacterium tuberculosis , the causative agent of tuberculosis, encodes many coiled-coil proteins that are homologous to DivIVA with a potential role in mycobacterial cell elongation. Here we describe studies on Mycobacterium smegmatis MSMEG_2416, a homologue of M. tuberculosis Rv2927c. Two previous independent studies showed that MSMEG_2416 was involved in septation (subsequently referred to as sepIVA). Contrary to these previous reports, we found sepIVA to be dispensable for growth in laboratory media by generating a viable null mutant. The mutant strain did, however, show a number of differences, including a change in colony morphology and biofilm formation that could be reversed on complementation with sepIVA as well as Rv2927c, the sepIVA homologue from M. tuberculosis . However, analysis of cell wall lipids did not reveal any alterations in lipid profiles of the mutant strain. Microscopic examination of the mutant revealed longer cells with more septa, which occurred at irregular intervals, often generating mini-compartments, a profile similar to that observed in the previous studies following conditional depletion, highlighting a role for sepIVA in mycobacterial growth.", }