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Volume 166, Issue 8

, a commensal of the oral microbiota of domestic cats, is a reservoir of transferrable antimicrobial resistance Free

Abstract

is a commensal bacterium from the oral microbiota of domestic cats, with a still obscure clinical importance. In this work, we analysed the ability of feline strains of to transfer antimicrobial resistance genes to isolated from humans through plasmids. To this end, we first analysed all publicly available genomes from cat staphylococci using computational methods to build a pan-resistome. Genes that encode resistance to erythromycin, gentamicin, mupirocin and tetracycline, common to human and cat staphylococci and previously described to be located in mobile genetic elements, were chosen for the next analyses. We studied 15 strains of , which were shown to be genetically different by GTG-PCR. As observed by disc diffusion, resistance to tetracycline was widespread (80 %), followed by resistance to erythromycin (40 %), gentamicin (27 %) and mupirocin (7 %). The strains were positive for several antimicrobial resistance genes and more than half of them harboured plasmids. The loss of plasmids and resistance genes in some strains were induced by stress with SDS. Through conjugation experiments, we observed that these plasmids can be transferred to , thus increasing its potential to resist drug therapy. Our findings show that , an underestimated inhabitant of the cat microbiota, can be a reservoir of antimicrobial resistance genes for and, like many other staphylococci, be an overlooked and silent threat to their animal hosts and humans living with them.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): feline , horizontal gene transfer , One Health , pan-resistome , plasmids and staphylococci
Funding
This study was supported by the:
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award Finance code 001 - 23038.002486/2018-26)
    • Principle Award Recipient: Ana Luisa Andrade-Oliveira
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award Finance Code 001 - 23038.002486/2018-26)
    • Principle Award Recipient: Ciro César Rossi
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 305940/2018-0)
    • Principle Award Recipient: Marcia Giambiagi-deMarval
  • Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (Award 26/201.451/2014)
    • Principle Award Recipient: Marcia Giambiagi-deMarval
  • Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (Award E-26/010.101056/2018)
    • Principle Award Recipient: Marcia Giambiagi-deMarval
  • Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (Award E-26/203.037/2016)
    • Principle Award Recipient: Marcia Giambiagi-deMarval
© 2020 The Authors
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2020-06-10
2024-04-24
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Staphylococcus nepalensis, a commensal of the oral microbiota of domestic cats, is a reservoir of transferrable antimicrobial resistance
Microbiology 166, 727 (2020); https://doi.org/10.1099/mic.0.000940
/content/journal/micro/10.1099/mic.0.000940
/content/journal/micro/10.1099/mic.0.000940
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