1887

Abstract

Outer-membrane vesicles (OMVs) produced by deliver bacterial components to host cells, provide a mechanism for stabilization of secreted components and may allow the bacteria to exert ‘long-range’ effects in the gastric niche, promoting persistence. In addition to their well-characterized host cell interactions, membrane vesicles improve stress survival in other bacterial species, and are constitutively produced by both pathogenic and non-pathogenic bacteria. We aimed to determine whether OMVs could improve survival of a range of stressors. The effects of purified OMVs on the resistance of to a range of environmental and antimicrobial stresses were determined using growth curves and survival assays. Addition of purified OMVs to cultures provided dose-dependent protection against hydrogen peroxide-mediated killing. Supplementation with OMVs also partially protected against the bactericidal effects of the antibiotics clarithromycin and levofloxacin, but not against amoxicillin nor metronidazole. Addition of purified OMVs allowed to grow in the presence of inhibitory concentrations of the antimicrobial peptide LL-37. In the presence of 50 µg OMVs ml, significantly enhanced growth was observed at higher LL-37 concentrations compared with lower LL-37 concentrations, suggesting that OMV–LL-37 interactions might facilitate release of growth-promoting nutrients. Taken together, these data indicate that production of membrane vesicles could help to survive exposure to antibiotics and host antimicrobial defences during infection.

Funding
This study was supported by the:
  • Lolwah Mohammad Alsharaf , Government of the State of Kuwait
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2020-05-28
2020-12-04
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