1887

Abstract

is a multidrug-resistant opportunistic pathogen that affects patients with a compromised immune system and is becoming increasingly important as a hospital-derived infection. This pathogen is difficult to treat owing to its intrinsic multidrug resistance and ability to form antimicrobial-tolerant biofilms. In the present study, we aimed to assess the potential use of zerumbone as a novel anti-biofilm and/or anti-virulence agent against . The results showed that zerumbone at sub-inhibitory doses decreased biofilm formation and disrupted established biofilms. The zerumbone-induced decrease in biofilm formation was dose-dependent based on the results of microtitre plate biofilm assays and confocal laser scanning microscopy. In addition, our data validated the anti-virulence efficacy of zerumbone, wherein it significantly interfered with the motility of . To support these phenotypic results, transcriptional analysis revealed that zerumbone downregulated the expression of biofilm- and virulence-associated genes (, , and ) in . Overall, our findings suggested that zerumbone might be a promising bioactive agent for the treatment of biofilm- and virulence-related infections caused by multidrug-resistant .

Funding
This study was supported by the:
  • Yong-Bin Eom , National Research Foundation of Korea , (Award NRF-2017R1D1A1B03032960)
  • Yong-Bin Eom , Soonchunhyang University , (Award SCH-20200312)
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/content/journal/micro/10.1099/mic.0.000930
2020-05-28
2020-09-20
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