RT Journal Article SR Electronic(1) A1 Gong, Yanshan A1 Yang, Yongsheng A1 Chen, Yan A1 Sun, Bingqing A1 Xue, Yafei A1 Xu, Xinxin A1 Wang, Xi A1 Islam, Nazrul A1 Du, Xiaoli A1 Hu, QinghaiYR 2020 T1 Characterization of the hemolytic activity of Riemerella anatipestifer JF Microbiology, VO 166 IS 5 SP 436 OP 439 DO https://doi.org/10.1099/mic.0.000896 PB Microbiology Society, SN 1465-2080, AB Riemerella anatipestifer infection causes serious economic losses in the duck industry worldwide. Acute septicemia and high blood bacterial loading in R. anatipestifer infected ducks indicate that R. anatipestifer may be able to obtain iron and other nutrients by lysing duck erythrocytes to support its rapid growth and proliferation in the blood. However, so far, little is known about the hemolytic activity of R. anatipestifer to duck erythrocytes. In this study, 29 of 52 R . anatipestifer strains showed hemolytic activity on duck blood agar, whereas all the tested dba+ (with hemolytic activity on duck blood agar) and dba− strains created pores in the duck red blood cells, with 4.35–9.03% hemolytic activity in a liquid hemolysis assay after incubation for 24 h. The concentrated culture supernatants of all the tested R. anatipestifer strains and the extracted outer membrane proteins (OMPs) from dba+ R. anatipestifer strains showed hemolytic activity on duck blood agar. These results, together with the median lethal dose (LD50) of some dba+ and dba- R. anatipestifer strains in ducklings, suggested that there was no direct relationship between the hemolytic capacity of R. anatipestifer on duck blood agar and its virulence., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000896