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Volume 166, Issue 4

Effect of four kinds of complexing iron on the process of iron uptake by Free

Abstract

Iron (Fe), which is a necessary micronutrient for algal growth, plays an important role in the physiological metabolism and enzymatic reactions of algae. This study aimed to investigate the absorption process of four kinds of complexing iron absorbed by . Results showed that the absorptive capacity of to complex iron was inversely proportional to the stability of the complex bond of complex iron. Complex iron with weak binding ability can be quickly adsorbed by . The absorptive rate was as follows: ferric humate, ferric oxalate >ammonium ferric citrate >EDTA Fe. For EDTA-Fe with a strong binding ability, a moderate iron concentration (e.g. 0.6 mg l) is favourable for iron uptake by . Our experiments also revealed that the process of separating iron from complex iron before entering algal cells was probably as follows: iron complexed with organic ligands were firstly adsorbed on the surface of algae cells; afterwards, iron ions were captured by organic matter on the surface of algae cells, accompanied by the rupture of the bond between Fe and ligand; finally, the Fe entered into the cell of algae while the organic ligands returned to the medium.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Complex iron, Anabaena. flos-aquae, absorption pathway, iron concentration
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 51708130)
    • Principle Award Recipient: Junxia Liu
  • National Natural Science Foundation of China (Award 51308131)
    • Principle Award Recipient: Zhihong Wang
© 2020 The Authors
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2020-02-05
2024-04-23
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References

  1. Qin B, Zhu G, Gao G, Zhang Y, Li W et al. A drinking water crisis in lake Taihu, China: linkage to climatic variability and lake management. Environ Manage 2010; 45:105–112 [View Article][PubMed]
     [Google Scholar]
  2. Huang J, Xu Q, Xi B, Wang X, Li W et al. Impacts of hydrodynamic disturbance on sediment resuspension, phosphorus and phosphatase release, and cyanobacterial growth in lake tai. Environ Earth Sci 2015; 74:3945–3954 [View Article]
     [Google Scholar]
  3. Le Manach S, Sotton B, Huet H, Duval C, Paris A et al. Physiological effects caused by microcystin-producing and non-microcystin producing Microcystis aeruginosa on medaka fish: A proteomic and metabolomic study on liver. Environmental Pollution 2018; 234:523–537 [View Article]
     [Google Scholar]
  4. Rueter JG, Petersen RR. Micronutrient effects on cyanobacterial growth and physiology. New Zeal J Mar Fresh 1987; 21:435–445 [View Article]
     [Google Scholar]
  5. Chappell PD, Moffett JW, Hynes AM, Webb EA. Molecular evidence of iron limitation and availability in the global diazotroph Trichodesmium. Isme J 2012; 6:1728–1739 [View Article][PubMed]
     [Google Scholar]
  6. Fujii M, Rose AL, Omura T, Waite TD, Manabu F. Effect of Fe(II) and Fe(III) transformation kinetics on iron acquisition by a toxic strain of Microcystis aeruginosa. Environ Sci Technol 2010; 44:1980–1986 [View Article][PubMed]
     [Google Scholar]
  7. Fraser JM, Tulk SE, Jeans JA, Campbell DA, Bibby TS et al. Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLoS One 2013; 8:e59861 [View Article]
     [Google Scholar]
  8. Xu H, Zhu G, Qin B, Paerl HW et al. Growth response of Microcystis spp. to iron enrichment in different regions of lake Taihu, China. Hydrobiologia 2013; 700:187–202 [View Article]
     [Google Scholar]
  9. Radic A, Lacan F, Murray JW. Iron isotopes in the seawater of the equatorial Pacific Ocean: new constraints for the oceanic iron cycle. Earth Planet Sci Lett 2011; 306:1–10 [View Article]
     [Google Scholar]
  10. Poulton SW, Raiswell R. Chemical and physical characteristics of iron oxides in riverine and glacial meltwater sediments. Chem Geol 2005; 218:203–221 [View Article]
     [Google Scholar]
  11. Wells ML, Zorkin NG, Lewis AG. The role of colloid chemistry in providing a source of iron to phytoplankton. J Mar Res 1983; 41:731–746 [View Article]
     [Google Scholar]
  12. Hutchins DA, Witter AE, Butler A, Luther GW. Competition among marine phytoplankton for different chelated iron species. Nature 1999; 400:858–861 [View Article]
     [Google Scholar]
  13. Norman L, Cabanesa DJE, Blanco-Ameijeiras S, Moisset SAM, Hassler CS. Iron biogeochemistry in aquatic systems: from source to bioavailability. Chimia 2014; 68:764–771 [View Article][PubMed]
     [Google Scholar]
  14. Kikuchi T, Fujii M, Terao K, Jiwei R, Lee YP et al. Correlations between aromaticity of dissolved organic matter and trace metal concentrations in natural and effluent waters: a case study in the Sagami River Basin, Japan. Sci Total Environ 2017; 576:36–45 [View Article][PubMed]
     [Google Scholar]
  15. Hassler CS, Norman L, Mancuso Nichols CA, Clementson LA, Robinson C et al. Iron associated with exopolymeric substances is highly bioavailable to oceanic phytoplankton. Mar Chem 2015; 173:136–147 [View Article]
     [Google Scholar]
  16. Pohl C, Fernández-Otero E. Iron distribution and speciation in oxic and anoxic waters of the Baltic sea. Mar Chem 2012; 145-147:1–15 [View Article]
     [Google Scholar]
  17. Morel F, Rueter J, Price N. Iron nutrition of phytoplankton and its possible importance in the ecology of ocean regions with high nutrient and low biomass. Oceanography 1991; 4:56–61 [View Article]
     [Google Scholar]
  18. Morel FMM, Kustka AB, Shaked Y. The role of unchelated Fe in the iron nutrition of phytoplankton. Limnol Oceanogr 2008; 53:400–404 [View Article]
     [Google Scholar]
  19. Gledhill M, van den Berg CMG, Berg C. Determination of complexation of iron(III) with natural organic complexing ligands in seawater using cathodic stripping voltammetry. Mar Chem 1994; 47:41–54 [View Article]
     [Google Scholar]
  20. Hider RC, Kong X. Chemistry and biology of siderophores. Nat Prod Rep 2010; 27:637–657 [View Article]
     [Google Scholar]
  21. Xing W, Huang W-min, Li D-hai, Liu Y-ding. Effects of iron on growth, pigment content, photosystem II efficiency, and siderophores production of Microcystis aeruginosa and Microcystis wesenbergii. Curr Microbiol 2007; 55:94–98 [View Article][PubMed]
     [Google Scholar]
  22. Itou Y, Okada S, Murakami M. Two structural isomeric siderophores from the freshwater cyanobacterium Anabaena cylindrica (NIES-19). Tetrahedron 2001; 57:9093–9099 [View Article]
     [Google Scholar]
  23. Dang TC, Fujii M, Rose AL, Bligh M, Waite TD. Characteristics of the freshwater cyanobacterium Microcystis aeruginosa grown in iron-limited continuous culture. Appl Environ Microbiol 2012; 78:1574–1583 [View Article][PubMed]
     [Google Scholar]
  24. Trick CG, Andersen RJ, Gillam A, Harrison PJ. Prorocentrin: an extracellular siderophore produced by the marine dinoflagellate Prorocentrum minimum. Science 1983; 219:306–308 [View Article]
     [Google Scholar]
  25. Rueter JG, Ohki K, Fujita Y. The effect of iron nutrition on photosynthesis and nitrogen fixation in cultures of trichodesmium (cyanophyceae)1. J Phycol 1990; 26:30–35 [View Article]
     [Google Scholar]
  26. Orlowska E, Roller A, Pignitter M, Jirsa F, Krachler R et al. Synthetic iron complexes as models for natural iron-humic compounds: synthesis, characterization and algal growth experiments. Sci Total Environ 2016; 40:94–104
     [Google Scholar]
  27. Weger HG, Matz CJ, Magnus RS, Walker CN, Fink MB et al. Differences between two green algae in biological availability of iron bound to strong chelators. Can J Bot 2006; 84:412400–411 [View Article]
     [Google Scholar]
  28. Kaushik MS, Srivastava M, Singh A, Mishra AK. Impairment of ntcA gene revealed its role in regulating iron homeostasis, ROS production and cellular phenotype under iron deficiency in cyanobacterium Anabaena sp. PCC 7120. World J Microbiol Biotechnol 2017; 33:158 [View Article][PubMed]
     [Google Scholar]
  29. Lammers PJ, Sanders-Loehr J. Active transport of ferric schizokinen in Anabaena sp. J Bacteriol 1982; 151:288294 [View Article][PubMed]
     [Google Scholar]
  30. Wilhelm SW, Trick CG. Iron-Limited growth of cyanobacteria: multiple siderophore production is a common response. Limnol Oceanogr 1994; 39:1979–1984 [View Article]
     [Google Scholar]
  31. Lammers PJ, Sandersloehr J. Active transport of ferrischizokinen in cyanobacteria.. In Saltman P, Hegenauer J. (editors) Biochemistry and physiology of iron : proceedings, Fifth International Conference on Proteins of Iron Storage and Transport, Univ of California, San Diego, August 24-26, 1981 1982
     [Google Scholar]
  32. Fu QL, Fujii M, Natsuike M, Waite TD. Iron uptake by bloom-forming freshwater cyanobacterium Microcystis aeruginosa in natural and effluent waters. Environ Pollut 2019; 247:392–400 [View Article][PubMed]
     [Google Scholar]
  33. Qiu Y, Wang Z, Liu F, Liu J, Zhou T. Effect of different kinds of complex iron on the growth of Anabaena flos-aquae. Environmental technology 20181–8
     [Google Scholar]
  34. Franklin NM, Stauber JL, Markich SJ, Lim RP. pH-dependent toxicity of copper and uranium to a tropical freshwater alga (Chlorella sp.). Aquat Toxicol 2000; 48:275–289 [View Article][PubMed]
     [Google Scholar]
  35. Chen M, Wang WX. Accelerated uptake by phytoplankton of iron bound to humic acids. Aquat. Biol. 2008; 3:155–166 [View Article]
     [Google Scholar]
  36. Robert S, Hugo B, Pierre-Louis B, Thibaut L, FranOis-Yves B et al. A comparative study of iron uptake mechanisms in marine microalgae: iron binding at the cell surface is a critical step. Plant Physiology 2012; 160:2271–2284
     [Google Scholar]
  37. Singh A, Mishra AK. Influence of various levels of iron and other abiotic factors on Siderophorogenesis in paddy field cyanobacterium Anabaena oryzae. Appl Biochem Biotechnol 2015; 176:372–386 [View Article]
     [Google Scholar]
  38. Shaked Y, Kustka AB, Morel FMM. A general kinetic model for iron acquisition by eukaryotic phytoplankton. Limnol Oceanogr 2005; 50:872–882 [View Article]
     [Google Scholar]
  39. Hu Q, Wang Z, Liu L. Effect of different valence iron on the growth of Scenedesmus quadricauda and its mechanism. Water & Wastewater 2014; 3:155–166
     [Google Scholar]
  40. Guo J, Annett AL, Taylor RL, Lapi S, Ruth TJ et al. COPPER-UPTAKE kinetics of coastal and oceanic DIATOMS1. J Phycol 2010; 46:1218–1228 [View Article]
     [Google Scholar]
  41. Shi G, Zhang F. Physical Chemistry of Water and Wastewater China: Machinery Industry Press; 2007
     [Google Scholar]
  42. Sonier MB, Contreras DA, Treble RG, Weger HG. Erratum: two distinct pathways for iron acquisition by iron-limited cyanobacterial cells: evidence from experiments using siderophores and synthetic chelators. Botany 2012; 90:1326–1327 [View Article]
     [Google Scholar]
  43. Hu Q. The study of regularity and mechanism of different valence iron on algal blooms Guangdong University of Technology; 2013
     [Google Scholar]
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Effect of four kinds of complexing iron on the process of iron uptake by Anabaena flos-aquae
Microbiology 166, 359 (2020); https://doi.org/10.1099/mic.0.000891
/content/journal/micro/10.1099/mic.0.000891
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