1887

Abstract

Duplication of the bacterial nucleoid is necessary for cell division hence specific arrest of DNA replication inhibits divisions culminating in filamentation, nucleoid dispersion and appearance of a-nucleated cells. It is demonstrated here that during the first 10 min however, enhanced residual divisions: the proportion of constricted cells doubled (to 40%), nucleoids contracted and cells remodelled dimensions: length decreased and width increased. The preliminary data provides further support to the existence of temporal and spatial couplings between the nucleoid/replisome and the sacculus/divisome, and is consistent with the idea that bacillary bacteria modulate width during the division process exclusively.

Funding
This study was supported by the:
  • Professor Arieh Zaritsky , United States - Israel Binational Science Foundation , (Award 2017004)
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2020-03-02
2020-06-02
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References

  1. Bazill GW. Lethal unbalanced growth in bacteria. Nature 1967; 216:346–349 [CrossRef]
    [Google Scholar]
  2. Donachie WD. Control of cell division in Escherichia coli: experiments with thymine starvation. J Bacteriol 1969; 100:260–268 [CrossRef]
    [Google Scholar]
  3. Ahmad SI, Kirk SH, Eisenstark A. Thymine metabolism and thymineless death in prokaryotes and eukaryotes. Annu Rev Microbiol 1998; 52:591–625 [CrossRef]
    [Google Scholar]
  4. Khodursky A, Guzmán EC, Hanawalt PC. Thymineless death lives on: new insights into a classic phenomenon. Annu Rev Microbiol 2015; 69:247–263 [CrossRef]
    [Google Scholar]
  5. Guzmán EC, Martín CM, death T. Thymineless death, at the origin. Front Microbiol 2015; 6:5 [CrossRef]
    [Google Scholar]
  6. Zaritsky A, Vollmer W, Männik J, Liu C. Does the Nucleoid Determine Cell Dimensions in Escherichia coli?. Front Microbiol 2019; 10:1717 [CrossRef]
    [Google Scholar]
  7. Martín CM, Guzmán EC. DNA replication initiation as a key element in thymineless death. DNA Repair 2011; 10:94–101 [CrossRef]
    [Google Scholar]
  8. Martín CM, Viguera E, Guzmán EC. Abortive initiations of chromosome replication under thymine starvation correlate with cell death in Escherichia coli. DNA Repair 2014; 18:10–17 [CrossRef]
    [Google Scholar]
  9. Thanbichler M. Synchronization of chromosome dynamics and cell division in bacteria. Cold Spring Harb Perspect Biol 2010; 2:a000331 [CrossRef]
    [Google Scholar]
  10. Zaritsky A, Woldringh CL. Chromosome replication, cell growth, division and shape: a personal perspective. Front Microbiol 2015; 6:756 [CrossRef]
    [Google Scholar]
  11. Helmstetter C, Cooper S, Pierucci O, Revelas E. On the bacterial life sequence. Cold Spring Harb Symp Quant Biol 1968; 33:809–822 [CrossRef]
    [Google Scholar]
  12. Jimenez-Sanchez A. Equations of a life. Algorithmic analysis of the bacterial cell cycle and the chromosome replication LAP Lambert Academic Publishing; 2018ISBN-13 978-613-9-57419-3
    [Google Scholar]
  13. Zaritsky A, Wang P, Vischer NOE. Instructive simulation of the bacterial cell division cycle. Microbiology 2011; 157:1876–1885 [CrossRef]
    [Google Scholar]
  14. Bremer H, Chuang L. Cell division after inhibition of chromosome replication in Escherichia coli. J Theor Biol 1981; 93:909–926 [CrossRef]
    [Google Scholar]
  15. Den Blaauwen T, Buddelmeijer N, Aarsman ME, Hameete CM, Nanninga N. Timing of FtsZ assembly in Escherichia coli. J Bacteriol 1999; 181:5167–5175 [CrossRef]
    [Google Scholar]
  16. Bigot S, Sivanathan V, Possoz C, Barre F-X, Cornet F. Ftsk, a literate chromosome segregation machine. Mol Microbiol 2007; 64:1434–1441 [CrossRef]
    [Google Scholar]
  17. Dubarry N, Barre F-X. Fully efficient chromosome dimer resolution in Escherichia coli cells lacking the integral membrane domain of FtsK. Embo J 2010; 29:597–605 [CrossRef]
    [Google Scholar]
  18. Meacock PA, Pritchard RH. Relationship between chromosome replication and cell division in a thymineless mutant of Escherichia coli B/r. J Bacteriol 1975; 122:931–942 [CrossRef]
    [Google Scholar]
  19. Powell EO. Growth rate and generation time of bacteria, with special reference to continuous culture. J Gen Microbiol 1956; 15:492–511 [CrossRef]
    [Google Scholar]
  20. Sueoka N, Yoshikawa H. The chromosome of Bacillus subtilis. I. theory of marker frequency analysis. Genetics 1965; 52:747–757
    [Google Scholar]
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