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Volume 166, Issue 3

Adaptation to pH stress by can affect its symbiosis with the Hawaiian bobtail squid () Open Access

Abstract

Many microorganisms engaged in host-microbe interactions pendulate between a free-living phase and a host-affiliated stage. How adaptation to stress during the free-living phase affects host-microbe associations is unclear and understudied. To explore this topic, the symbiosis between Hawaiian bobtail squid () and the luminous bacterium was leveraged for a microbial experimental evolution study. experienced adaptation to extreme pH while apart from the squid host. was serially passaged for 2000 generations to the lower and upper pH growth limits for this microorganism, which were pH 6.0 and 10.0, respectively. was also serially passaged for 2000 generations to vacillating pH 6.0 and 10.0. Evolution to pH stress both facilitated and impaired symbiosis. Microbial evolution to acid stress promoted squid colonization and increased bioluminescence for , while symbiont adaptation to alkaline stress diminished these two traits. Oscillatory selection to acid and alkaline stress also improved symbiosis for , but the facilitating effects were less than that provided by microbial adaptation to acid stress. In summary, microbial adaptation to harsh environments amid the free-living phase may impact the evolution of host-microbe interactions in ways that were not formerly considered.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bioluminescence , host-microbe interactions , Microbial experimental evolution , mutualism and symbiosis
Funding
This study was supported by the:
  • College of William and Mary Startup (Award 120819)
    • Principle Award Recipient: William Soto
  • College of William and Mary Startup (Award 121089)
    • Principle Award Recipient: William Soto
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2020-01-22
2024-04-24
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Adaptation to pH stress by Vibrio fischeri can affect its symbiosis with the Hawaiian bobtail squid (Euprymna scolopes)
Microbiology 166, 262 (2020); https://doi.org/10.1099/mic.0.000884
/content/journal/micro/10.1099/mic.0.000884
/content/journal/micro/10.1099/mic.0.000884
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