RT Journal Article SR Electronic(1) A1 Juntachai, Weerapong A1 Chaichompoo, Athipat A1 Chanarat, SittinanYR 2020 T1 Ambient pH regulates secretion of lipases in Malassezia furfur JF Microbiology, VO 166 IS 3 SP 288 OP 295 DO https://doi.org/10.1099/mic.0.000879 PB Microbiology Society, SN 1465-2080, AB Malassezia is a lipophilic cutaneous commensal yeast and associated with various skin disorders. The yeast also causes bloodstream infection via intravascular catheters and can be detected even in human gut microbiota. Ambient pH is one of the major factors that affect the physiology and metabolism of several pathogenic microorganisms. Although dynamic changes of pH environment in different parts of the body is a great challenge for Malassezia to confront, the role that ambient pH plays in Malassezia is largely unknown. In this study, we investigated the impact of ambient pH on physiology and expression of lipases in M. furfur grown under different pH conditions. The yeast was able to grow in media ranging from pH 4 to 10 without morphological alteration. Elevation in pH value enhanced the extracellular lipase activity but decreased that of intracellular lipase. The qPCR results revealed that a set of functional lipase genes, LIP3-6, were constitutively expressed regardless of pH conditions or exposure time. Based on the data, we conclude that the external pH plays a promotional role in the secretion of lipases but exerts less effect on transcription of the genes and morphology in M. furfur., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000879