1887

Abstract

The virulence genes of are modulated during infection by several regulatory systems, and the RcsCDB system is one of the most important of these. The . Typhimurium EG14873 () strain harbours the point mutation, displaying a constitutive activation of this system, which is characterized by mucoid colonies and attenuated virulence phenotypes. In this work, the stability of the mutation was analysed under stress conditions. Under acid and anaerobic stresses, we observed the appearance of small and non-mucoid colonies of the strain. The sequencing of the gene from these colonies showed that the mutation is conserved. Moreover, we found that small colonies were also generated when the wild-type strain grew in acid and anaerobic conditions. It is worth noting that the transition from normal to atypical colonies of both strains only took place after several days of incubation and was not observed during eukaryotic cell infection. Therefore, the appearance of these atypical colonies is a characteristic feature of . Typhimurium strains under stressful situations and does not involve a reversion of the allele and nor does it imply any risk to mammalian cells. Therefore, we propose that the . Typhimurium strain is a good candidate for the development of attenuated vaccines.

Keyword(s): Salmonella , infection and vaccine
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/content/journal/micro/10.1099/mic.0.000873
2019-11-12
2019-12-11
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