1887

Microbiology

Prevalence and molecular characterization of non-tuberculous mycobacteria in hospital soil and dust of a developing country, Iran No Access

Abstract

The presence and diversity of mycobacteria that are capable of survival in a harsh and adverse condition, such as hospital environments, have not been comprehensively studied. This study aimed to assess the frequency and diversity of mycobacteria in hospital soil and dust of a developing country using a combination of molecular and conventional methods. A total of 318 hospital dust and soil samples collected from 38 hospitals were analysed using standard protocols for characterization of mycobacteria. The conventional tests were used for preliminary identification and Runyon’s classification, the PCR amplification of the 65 gene and sequence analyses of 16SrRNA were applied for genus and species identification. In total, 28 samples (8.8 %) were positive for mycobacteria. The isolates included 33 mycobacteria species including 19 rapidly growing and 14 slowly growing organisms. The most prevalent species were and five isolates (15.1 %) each, four isolates (12.12 %) and and complex three isolates (9.1 %) each and two isolates (6 %) each. The remaining isolates consisted the single strains of eight various mycobacterium species, the results of our study revealed that soil and dust in hospitals can be the reservoir of mycobacteria. This reaffirms the fact that these organisms due to intrinsic resistance can persist in hospitals and create a threat to patient’s health, in particular to those who suffer from weakness of immunity.

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Keyword(s): hospital infection , 16SrRNA sequencing and non-tuberculous mycobacteria
© 2019 The Authors
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/content/journal/micro/10.1099/mic.0.000857
2019-10-15
2019-11-21
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.000857
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Prevalence and molecular characterization of non-tuberculous mycobacteria in hospital soil and dust of a developing country, Iran
Microbiology Society , mic000857; https://doi.org/10.1099/mic.0.000857
/content/journal/micro/10.1099/mic.0.000857
/content/journal/micro/10.1099/mic.0.000857
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