1887

Abstract

To investigate the metabolism of 18:2-6 and 18:3-3 by pure cultures of , two different strains (RL 1 and ST18) were each incubated in the presence of 40 µg ml 18:2-6 or 18:3-3. Pure cultures of D1 and P18 were included as control treatments. Similar to the metabolism of , both strains converted 18:2-6 or 18:3-3 to -9, -11 CLA or -9, -11, -15 CLnA, after which it was further reduced to -11 18:1 or -11, -15 18:2, respectively. additionally reduced -11 18:1 to 18:0. -11, -15 18:2 was also further metabolized by , although -11 18:1 did not accumulate, and only minor amounts of 18:0 were formed. The time frame of 18:2-6 and 18:3-3 biohydrogenation by was comparable with , indicating that and might be alternative biohydrogenators of 18:2-6 and 18:3-3 in the rumen.

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2019-07-01
2020-11-26
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