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Volume 165, Issue 3

High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content Free

Abstract

The human pathogen Pseudomonas aeruginosa can cause both acute infections and chronic biofilm-based infections. Expression of acute virulence factors is positively regulated by cAMP, whereas biofilm formation is positively regulated by c-di-GMP. We provide evidence that increased levels of cAMP, caused by either a lack of degradation or increased production, inhibit P. aeruginosa biofilm formation. cAMP-mediated inhibition of P. aeruginosa biofilm formation required Vfr, and involved a reduction of the level of c-di-GMP, as well as reduced production of biofilm matrix components. A mutant screen and characterization of defined knockout mutants suggested that a subset of c-di-GMP-degrading phosphodiesterases is involved in cAMP-Vfr-mediated biofilm inhibition in P. aeruginosa .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm formation , c-di-GMP , cAMP , Pseudomonas aeruginosa and virulence factors
© 2019 The Authors
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2019-01-21
2024-04-25
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High levels of cAMP inhibit Pseudomonas aeruginosa biofilm formation through reduction of the c-di-GMP content
Microbiology 165, 324 (2019); https://doi.org/10.1099/mic.0.000772
/content/journal/micro/10.1099/mic.0.000772
/content/journal/micro/10.1099/mic.0.000772
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