RT Journal Article SR Electronic(1) A1 Nishino, Koki A1 Morita, Yusuke A1 Takahashi, Sawako A1 Okumura, Mai A1 Shiratani, Shusaku A1 Umemura, Kosuke A1 Narumi, Issay A1 Kondo, Chie A1 Ochiai, Ryosuke A1 Oshima, Taku A1 Nishida, HiromiYR 2018 T1 Enlargement of Deinococcus grandis spheroplasts requires Mg2+ or Ca2+ JF Microbiology, VO 164 IS 11 SP 1361 OP 1371 DO https://doi.org/10.1099/mic.0.000716 PB Microbiology Society, SN 1465-2080, AB While the cell wall strictly controls cell size and morphology in bacteria, spheroplasts lack cell walls and can become enlarged in growth medium under optimal conditions. Optimal conditions depend on the bacterial species. We frequently observed extreme enlargement of spheroplasts of the radiation-resistant bacterium Deinococcus grandis in Difco Marine Broth 2216, but not in TGY broth (a commonly used growth medium for Deinococcus). Thorough investigation of media components showed that the presence of Mg2+ or Ca2+ promoted extreme spheroplast enlargement, synthesizing the outer membrane. Our findings strongly suggest that Mg2+ or Ca2+ enlarges spheroplasts, which could change the lipid composition of the spheroplast membrane., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000716