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Volume 164, Issue 9

Oxyresveratrol-induced DNA cleavage triggers apoptotic response in Candida albicans Free

Abstract

Oxyresveratrol is a naturally occurring phytoalexin produced by plants in response to infection. Biological activities of oxyresveratrol have been studied such as antioxidant, anticancer and anti-inflammation. However, further antimicrobial activity and its mechanism need to be investigated. This study exhibited growth inhibition against pathogenic fungi and investigated its mode of action. Oxyresveratrol inflicted cleavage on DNA, leading to G2/M phase arrest. DNA damage by oxyresveratrol was not the result of oxidative stress but it was triggered by direct binding to DNA. Oxyresveratrol-treated cells showed an apoptotic pathway characterized by phosphatidylserine exposure, apoptotic volume decrease and metacaspase activation. Mitochondria-associated apoptotic features also appeared. Oxyresveratrol-induced Ca overload led to mitochondrial membrane depolarization and release of cytochrome c from mitochondria to cytosol. In conclusion, oxyresveratrol with DNA-binding affinity induces DNA cleavage, and eventually leads to mitochondria-mediated apoptosis in Candida albicans.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): apoptosis , Candida albicans , DNA cleavage and oxyresveratrol
© 2018 The Authors
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2018-07-19
2024-04-20
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Oxyresveratrol-induced DNA cleavage triggers apoptotic response in Candida albicans
Microbiology 164, 1112 (2018); https://doi.org/10.1099/mic.0.000696
/content/journal/micro/10.1099/mic.0.000696
/content/journal/micro/10.1099/mic.0.000696
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