1887

Abstract

How DNA metabolism is adapted to survival of organisms such as the bacterium SS9 at high pressure is unknown. Previously, a high pressure-sensitive SS9 transposon mutant (FL31) was identified, with an insertion in a putative gene. The RctB protein is essential for replication initiation at the origin of chromosome II, . Using a plasmid-based system in we have identified the replication origin of chromosome II from SS9 and have shown that the putative gene, disrupted in FL31, is essential for function. Moreover, we found that a region corresponding to the incompatibility region () exerts an inhibitory effect on . The truncated gene in FL31 confers insensitivity to inhibition, indicating that the C-terminus of RctB is important for the negative regulation of replication. The RctB proteins of and are partially interchangeable, but full functionality is achieved only with the cognate origin. Our findings provide the first characterization of the replication origin of chromosome II in a deep-sea bacterium.

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2018-06-01
2024-03-28
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