1887

Abstract

As a master regulator, the alternative sigma factor RpoS coordinates the transcription of genes associated with protection against environmental stresses in bacteria. In , RpoS is also involved in quorum sensing and virulence. The cellular RpoS level is regulated at multiple levels, whereas the post-transcriptional regulation of rpoS in remains unclear. To identify and characterize small regulatory RNAs (sRNAs) regulating RpoS in , an sRNA library expressing a total of 263 sRNAs was constructed to examine their regulatory roles on expression. Our results demonstrate that expression is repressed by the RpoS-dependent sRNA RgsA at the post-transcriptional level. Unlike OxyS, an sRNA previously known to repress expression under oxidative stress in , RgsA represses expression during the exponential phase. This repression requires the RNA chaperone Hfq. Furthermore, the 71–77 conserved region of RgsA is necessary for full repression of expression, and the −25 to +27 region of rpoS mRNA is sufficient for RgsA-mediated repression. Together, our results not only add RgsA to the RpoS regulatory circuits but also highlight the complexity of interplay between sRNAs and transcriptional regulators in bacteria.

Keyword(s): Hfq , Pseudomonas aeruginosa , RgsA , RpoS and small RNA
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2018-04-01
2020-04-01
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