Polyamines are required for normal growth in Free

Abstract

Polyamines (PAs) are ubiquitous polycations derived from basic -amino acids whose physiological roles are still being defined. Their biosynthesis and functions in nitrogen-fixing rhizobia such as have not been extensively investigated. Thin layer chromatographic and mass spectrometric analyses showed that Rm8530 produces the PAs, putrescine (Put), spermidine (Spd) and homospermidine (HSpd), in their free forms and norspermidine (NSpd) in a form bound to macromolecules. The genome encodes two putative ornithine decarboxylases (ODC) for Put synthesis. Activity assays with the purified enzymes showed that ODC2 (SMc02983) decarboxylates both ornithine and lysine. ODC1 (SMa0680) decarboxylates only ornithine. An mutant was similar to the wild-type in ODC activity, PA production and growth. In comparison to the wild-type, an mutant had 45 % as much ODC activity and its growth rates were reduced by 42, 14 and 44 % under non-stress, salt stress or acid stress conditions, respectively. The mutant produced only trace levels of Put, Spd and HSpd. Wild-type phenotypes were restored when the mutant was grown in cultures supplemented with 1 mM Put or Spd or when the gene was introduced gene expression was increased under acid stress and reduced under salt stress and with exogenous Put or Spd. An double mutant had phenotypes similar to the mutant. These results indicate that ODC2 is the major enzyme for Put synthesis in and that PAs are required for normal growth .

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2018-04-01
2024-03-29
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