1887

Abstract

Ornithine lipids (OLs) are phosphorus-free lipids found in many bacteria grown under phosphate deprivation, a condition that activates the PhoBR system and leads to phosphate uptake and metabolism. Two OL synthesis pathways have already been described. One depends on OlsB and OlsA acyltransferases to add, respectively, the first and second acyl chains to an ornithine molecule. The other pathway is carried out by OlsF, a bifunctional enzyme responsible for both acylation steps. Although lacks genes, an homologue () was identified in its genome. In this work we demonstrated that produces OLs and expresses in response to phosphate depletion, in a PhoBR-dependent manner. In , under similar condition, expression leads to OL accumulation. These results indicate a strong connection between OL synthesis and VC0489 from and, for the first time, a direct regulation of an homologue by the PhoBR system.

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2018-03-01
2024-03-29
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