The fission yeast Mtf2 is required for mitochondrial gene expression Free

Abstract

Mitochondrial gene expression is essential for adenosine triphosphate synthesis via oxidative phosphorylation, which is the universal energy currency of cells. Here, we report the identification and characterization of a homologue of Mtf2 (also called Nam1) in . The Δ mutant with the intron-containing mitochondrial DNA (mtDNA) exhibited impaired growth on a rich medium containing the non-fermentable carbon source glycerol, suggesting that is involved in mitochondrial function. deletion in a mitochondrial intron-containing background resulted in a barely detectable level of the mRNA and a reduction in the level of the mRNA, and severely impaired translation. In contrast, deletion in a mitochondrial intron-less background did not affect the levels of and mRNAs. However, Cox1 synthesis could not be restored to the control level in the Δ mutant with intron-less mtDNA. Our results suggest that unlike its counterpart in which plays a general role in synthesis of mtDNA-encoded proteins, Mtf2 primarily functions in translation and the effect of deletion on splicing of introns in mtDNA is likely due to a deficiency in the synthesis of intron-encoded maturases.

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2018-03-01
2024-03-29
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