1887

Microbiology

Volume 164, Issue 1

Molecular cloning and overexpression of , an acyl-CoA-dependent diacylglycerol acyltransferase, in the oleaginous yeast DMKU-RK253 Free

Abstract

Triacylglycerol (TAG) is a major component of lipid storage in yeast. The acyl CoA: diacylgycerol acyltransferase (DGAT) that catalyzes the final and rate-limiting step in the production of TAG is rather interesting. Consequently, cloning and analysis of the gene-encoding TAG synthase, diacylglycerol acyltransferase gene (), of the oleaginous yeast DMKU-RK253 were undertaken. Analysis of the deduced amino acid sequence of DGA1 from DMKU-RK253 () showed similarity with the acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) from other organisms. The cDNA of was cloned into the yeast expression vector pYES2 and heterologously overexpressed in . One of the transformants showed a 1.6-fold increase in lipid content compared with the wild-type strain harbouring the pYES2 empty vector. Furthermore, overexpression in DMKU-RK253 resulted in a 2.5-fold increase in lipid content when compared with the wild-type strain, and no significant differences in fatty acid composition were observed between -overexpressed and wild-type strains. Taken together, our results supported our hypothesis that the is a genetic factor that can be used for the development of a strain with improved lipid accumulation capabilities.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): diacylglycerol acyltransferase gene , lipid accumulation , oleaginous yeast , Rhodosporidiobolus fluvialis and Saccharomyces cerevisiae
© 2018 The Authors
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2018-01-01
2021-03-07
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Molecular cloning and overexpression of DGA1, an acyl-CoA-dependent diacylglycerol acyltransferase, in the oleaginous yeast Rhodosporidiobolus fluvialis DMKU-RK253
Microbiology 164, 1 (2018); https://doi.org/10.1099/mic.0.000584
/content/journal/micro/10.1099/mic.0.000584
/content/journal/micro/10.1099/mic.0.000584
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