1887

Abstract

Triacylglycerol (TAG) is a major component of lipid storage in yeast. The acyl CoA: diacylgycerol acyltransferase (DGAT) that catalyzes the final and rate-limiting step in the production of TAG is rather interesting. Consequently, cloning and analysis of the gene-encoding TAG synthase, diacylglycerol acyltransferase gene (), of the oleaginous yeast DMKU-RK253 were undertaken. Analysis of the deduced amino acid sequence of DGA1 from DMKU-RK253 () showed similarity with the acyl-CoA:diacylglycerol acyltransferase 2 (DGAT2) from other organisms. The cDNA of was cloned into the yeast expression vector pYES2 and heterologously overexpressed in . One of the transformants showed a 1.6-fold increase in lipid content compared with the wild-type strain harbouring the pYES2 empty vector. Furthermore, overexpression in DMKU-RK253 resulted in a 2.5-fold increase in lipid content when compared with the wild-type strain, and no significant differences in fatty acid composition were observed between -overexpressed and wild-type strains. Taken together, our results supported our hypothesis that the is a genetic factor that can be used for the development of a strain with improved lipid accumulation capabilities.

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2018-01-01
2024-03-28
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